Toxicological evaluations of betulinic acid and ursolic acid; common constituents of Houttuynia cordata used as an anthelmintic by the Naga tribes in North-east India

• Published in: Future journal of pharmaceutical sciences 2021-02, Vol.7 (1), p.39-13, Article 39
• Main Authors: Mishra, Vijaya, Soren, Amar Deep, Yadav, Arun Kumar
• Format: Article
• Language: English
• Subjects: Acids; Alzheimer's disease; Anesthesia; Betulinic acid, Phytocompounds, Toxicity, Ursolic acid; Drug dosages; Experiments; Food; Hematology; Laboratory animals; Malaria; Medicine; Medicine & Public Health; Mortality; Oral administration; Pharmaceutical sciences; Toxicity; Variance analysis
• ISSN: 2314-7253; 2314-7245; 2314-7253
• DOI: 10.1186/s43094-020-00173-4

Background Betulinic acid (BA) and ursolic acid (UA) are two major phytoconstituents of Houttuynia cordata Thunb., (Saururaceae) which is used as an anthelmintic in the traditional medicine system of the Nagas in Nagaland, India. This study evaluates their toxic potentials using rodent models (Swiss albino mice and Wistar rats) according to the OECD (Organization for Economic Cooperation and Development) guidelines. Acute and 28-day sub-acute oral toxicity studies were conducted, and evaluations were made based on biochemical, hematological, and histopathological observations. Results Acute oral toxicity study revealed the oral LD50 of both the test compounds to be > 2000 mg/kg in mice. Sub-acute administration of BA at 10 mg/kg body weight (b.w.) revealed a significant increase in serum glutamic oxaloacetic transaminase (SGOT), alkaline phosphatase (ALP), urea concentrations and eosinophil and lymphocyte counts in rats. Animals administered with 10 mg/kg b.w. UA revealed elevated neutrophil count, SGOT, ALP, and urea concentrations, whereas white blood cells (WBC), lymphocyte, and platelet counts were found to be low. Histopathological examinations of body organs revealed alterations in the architecture of the liver, kidney, and spleen tissues. Notably, all these alterations were recoverable as evident in the satellite group, indicating a recovering pattern from the toxic effects caused by the oral administration of these phytocompounds. Conclusion Although UA and BA possess several therapeutic properties, their long-term usage can cause mild toxicity in their users. This study also paves way for evaluating the optimum effective and safe dose of these phytocompounds.