Metatranscriptomic and Thermodynamic Insights into Medium-Chain Fatty Acid Production Using an Anaerobic Microbiome

Biomanufacturing from renewable feedstocks can offset fossil fuel-based chemical production. One potential biomanufacturing strategy is production of medium-chain fatty acids (MCFA) from organic feedstocks using either pure cultures or microbiomes. While the set of microbes in a microbiome can often...

Full description

Saved in:
Bibliographic Details
Published in:mSystems 2018-11, Vol.3 (6)
Main Authors: Scarborough, Matthew J, Lawson, Christopher E, Hamilton, Joshua J, Donohue, Timothy J, Noguera, Daniel R
Format: Article
Language:English
Subjects:
anaerobic digestion
Applied and Environmental Science
BASIC BIOLOGICAL SCIENCES
biorefining
carboxylate platform
Editor's Pick
hexanoic acid
medium-chain fatty acids
metagenomics
metatranscriptomics
octanoic acid
Research Article
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Biomanufacturing from renewable feedstocks can offset fossil fuel-based chemical production. One potential biomanufacturing strategy is production of medium-chain fatty acids (MCFA) from organic feedstocks using either pure cultures or microbiomes. While the set of microbes in a microbiome can often metabolize organic materials of greater diversity than a single species can and while the role of specific species may be known, knowledge of the carbon and energy flow within and between organisms in MCFA-producing microbiomes is only now starting to emerge. Here, we integrated metagenomic, metatranscriptomic, and thermodynamic analyses to predict and characterize the metabolic network of an anaerobic microbiome producing MCFA from organic matter derived from lignocellulosic ethanol fermentation conversion residue. A total of 37 high-quality (>80% complete,
ISSN:2379-5077
2379-5077
DOI:10.1128/MSYSTEMS.00221-18