Conversion of Escherichia coli into Mixotrophic CO2 Assimilation with Malate and Hydrogen Based on Recombinant Expression of 2-Oxoglutarate:Ferredoxin Oxidoreductase Using Adaptive Laboratory Evolution

We report the mixotrophic growth of Escherichia coli based on recombinant 2-oxoglutarate:ferredoxin oxidoreductase (OGOR) to assimilate CO2 using malate as an auxiliary carbon source and hydrogen as an energy source. We employ a long-term (~184 days) two-stage adaptive evolution to convert heterotro...

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Bibliographic Details
Published in:Microorganisms (Basel) 2023-01, Vol.11 (2), p.253
Main Authors: Cheng, Yu-Chen, Huang, Wei-Han, Lo, Shou-Chen, Huang, Eugene, Chiang, En-Pei Isabel, Huang, Chieh-Chen, Yang, Ya-Tang
Format: Article
Language:English
Subjects:
2-oxoglutarate:ferredoxin oxidoreductase
adaptive laboratory evolution
Assimilation
Carbon
Carbon dioxide
carbon fixation
Carbon sources
Cloning
Communication
Conversion
E coli
Energy sources
Enzymes
Escherichia coli
Evolution
Experiments
Ferredoxin
Genes
Genomes
Hydrogen
Ketoglutaric acid
Laboratories
Malate
Microorganisms
Mixotrophy
Oxidoreductase
reductive tricarboxylic acid cycle
Substrates
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Summary:We report the mixotrophic growth of Escherichia coli based on recombinant 2-oxoglutarate:ferredoxin oxidoreductase (OGOR) to assimilate CO2 using malate as an auxiliary carbon source and hydrogen as an energy source. We employ a long-term (~184 days) two-stage adaptive evolution to convert heterotrophic E. coli into mixotrophic E. coli. In the first stage of evolution with serine, diauxic growth emerges as a prominent feature. At the end of the second stage of evolution with malate, the strain exhibits mixotrophy with CO2 as an essential substrate for growth. We expect this work will open new possibilities in the utilization of OGOR for microbial CO2 assimilation and future hydrogen-based electro-microbial conversion.
ISSN:2076-2607
2076-2607
DOI:10.3390/microorganisms11020253