Gene Expression Analysis of Microtubers of Potato Solanum tuberosum L. Induced in Cytokinin Containing Medium and Osmotic Stress

Potato microtuber productions through in vitro techniques are ideal propagules for producing high quality seed potatoes. Microtuber development is influenced by several factors, i.e., high content sucrose and cytokinins are among them. To understand a molecular mechanism of microtuberization using o...

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Bibliographic Details
Published in:Plants (Basel) 2021-04, Vol.10 (5), p.876
Main Authors: Herrera-Isidron, Lisset, Valencia-Lozano, Eliana, Rosiles-Loeza, Pablo Yamild, Robles-Hernández, Maria Guadalupe, Napsuciale-Heredia, Abigail, Cabrera-Ponce, Jose Luis
Format: Article
Language:English
Subjects:
Carbon
cytokinin signaling
Cytokinins
Darkness
Gene expression
Gene regulation
Genomes
In vitro methods and tests
Kinases
Metabolism
microtuberization
Osmosis
Osmotic stress
potato
Potatoes
Propagules
Protein biosynthesis
Protein synthesis
Protein turnover
Proteins
Signal transduction
Signalling systems
Solanum tuberosum
Sucrose
two component system
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Summary:Potato microtuber productions through in vitro techniques are ideal propagules for producing high quality seed potatoes. Microtuber development is influenced by several factors, i.e., high content sucrose and cytokinins are among them. To understand a molecular mechanism of microtuberization using osmotic stress and cytokinin signaling will help us to elucidate this process. We demonstrate in this work a rapid and efficient protocol for microtuber development and gene expression analysis. Medium with high content of sucrose and gelrite supplemented with 2iP as cytokinin under darkness condition produced the higher quantity and quality of microtubers. Gene expression analysis of genes involved in the two-component signaling system ( ), cytokinin signaling, ( , , ) homeodomains ( , , ), auxin signaling, , carbon metabolism ( , , protein synthesis, and a morphogenetic regulator of tuberization ( ) was performed by qPCR real time. Differential gene expression was observed during microtuber development. Gene regulation of two component and cytokinin signaling is taking place during this developmental process, yielding more microtubers. Further analysis of each component is required to elucidate it.
ISSN:2223-7747
2223-7747
DOI:10.3390/plants10050876