One-Step Formation of Chondrocytes through Direct Reprogramming via Polysaccharide-Based Gene Delivery

An innovative strategy for the generation of chondrocytes was thoroughly studied in this paper. Polyetherimide-modified polysaccharides of Porphyra yezoensis (pmPPY) served as a nonviral gene vector and delivered Sox9 plasmid to directly reprogram mouse embryonic fibroblasts into chondrocytes. The g...

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Bibliographic Details
Published in:Advances in polymer technology 2019-01, Vol.2019 (2019), p.1-12
Main Authors: Xu, Ximing, Adu Frimpong, Michael, Deng, Wenwen, Cao, Xia, Wang, Yan, Yu, Qingtong, Yu, Jiangnan
Format: Article
Language:English
Subjects:
Biomedical materials
Calcium phosphates
Cartilage
Collagen
Cytotoxicity
Deoxyribonucleic acid
DNA
Enzyme-linked immunosorbent assay
Ethylenediaminetetraacetic acid
Fibroblasts
Genes
Genetic vectors
Infection
Laboratories
Methylene blue
Nanoparticles
Penicillin
Polyetherimide
Polyetherimides
Polysaccharides
Porphyra
Rheumatology
Stem cells
Tissue engineering
Toluidine blue
Toxicity
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Summary:An innovative strategy for the generation of chondrocytes was thoroughly studied in this paper. Polyetherimide-modified polysaccharides of Porphyra yezoensis (pmPPY) served as a nonviral gene vector and delivered Sox9 plasmid to directly reprogram mouse embryonic fibroblasts into chondrocytes. The gene transfer efficiency was evaluated through ELISA, RT-PCR, and Western blot. The induced chondrocytes were identified through toluidine blue, Safranin O, and the immunostaining. The expression level of collagen II was finally evaluated through western blot. The pSox9/pmPPY nanoparticles (1:50) showed lower cytotoxicity as well as greater gene transfection efficiency than Lipofectamine 2000 and polyetherimide (PEI) (p
ISSN:0730-6679
1098-2329
DOI:10.1155/2019/7632873