New fluorimetric assay of horseradish peroxidase using sesamol as substrate and its application to EIA

Horseradish peroxidase (HRP) is generally used as a label enzyme in enzyme immunoassay (EIA).The procedure used for HRP detection in EIA is critical for sensitivity and precision.This paper describes a novel fluorimetric assay for horseradish peroxidase (HRP) using sesamol as substrate.The principle...

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Bibliographic Details
Published in:Journal of pharmaceutical analysis 2012-04, Vol.2 (2), p.156-159
Main Authors: Arakawa, Hidetoshi, Nakabayashi, Shigeo, Ohno, Ken-ichi, Maeda, Masako
Format: Article
Language:English
Subjects:
Enzyme immunoassay (EIA)
Fluorescence
Horseradish peroxidase (HRP)
Sesamol
Thyroid stimulating hormone (TSH)
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Summary:Horseradish peroxidase (HRP) is generally used as a label enzyme in enzyme immunoassay (EIA).The procedure used for HRP detection in EIA is critical for sensitivity and precision.This paper describes a novel fluorimetric assay for horseradish peroxidase (HRP) using sesamol as substrate.The principle of the assay is as follow:sesamol (3,4-methylenedioxy phenol) is reacted enzymatically in the presence of hydrogen peroxide to produce dimeric sesamol.The dimer is fluorescent and can be detected sensitively at ...
ISSN:2095-1779
2214-0883
DOI:10.1016/j.jpha.2012.01.004