The antagonism mechanism of astilbin against cadmium-induced injury in chicken lungs via Treg/Th1 balance signaling pathway

• Published in: Ecotoxicology and environmental safety 2024-06, Vol.277, p.116364-116364, Article 116364
• Main Authors: Shi, Guangliang, Tai, Tiange, Miao, Yusong, Yan, Liangchun, Han, Tianyu, Dong, Han, Liu, Zhaoyang, Cheng, Tingting, Liu, Yiding, Yang, Yu, Fei, Shanshan, Pang, Bo, Chen, Tiezhu
• Format: Article
• Language: English
• Subjects: Animals; Astilbin; Cadmium; Cadmium - toxicity; Chickens; Flavonols - pharmacology; Inflammation; Lung - drug effects; Lung - pathology; Lung Injury - chemically induced; Lung Injury - drug therapy; Oxidative stress; Oxidative Stress - drug effects; Signal Transduction - drug effects; T-Lymphocytes, Regulatory - drug effects; Treg/Th1
• ISSN: 0147-6513; 1090-2414
• DOI: 10.1016/j.ecoenv.2024.116364

The purpose of this study was to investigate the effect of Treg/Th1 imbalance in cadmium-induced lung injury and the potential protective effect of astilbin against cadmium-induced lung injury in chicken. Cadmium exposure significantly decreased T-AOC and GSH-Px levels and SOD activity in the chicken lung tissues. In contrast, it significantly increased the MDA and NO levels. These results indicate that cadmium triggers oxidative stress in lungs. Histopathological analysis revealed that cadmium exposure further induced infiltration of lymphocytes in the chicken lungs, indicating that cadmium causes pulmonary damage. Further analysis revealed that cadmium decreased the expression of IL-4 and IL-10 but increased those of IL-17, Foxp3, TNF-α, and TGF-β, indicating that the exposure of cadmium induced the imbalance of Treg/Th1. Moreover, cadmium adversely affected chicken lung function by activating the NF-kB pathway and inducing expression of genes downstream to these pathways (COX-2, iNOS), associated with inflammatory injury in the lung tissue. Astilbin reduced cadmium-induced oxidative stress and inflammation in the lungs by increasing antioxidant enzyme activities and restoring Treg/Th1 balance. In conclusion, our results suggest that astilbin treatment alleviated the effects of cadmium-mediated lung injury in chickens by restoring the Treg/Th1 balance. [Display omitted] •Cadmium exposure triggers Treg/Th1 imbalanced and leads to inflammation.•Cadmium can cause inflammation via NF-kB pathway in chicken.•Astilbin regulates inflammation induced by Cd through Treg/Th1 signaling pathway.