Global Phenotypic Characterization of Effects of Fluoroquinolone Resistance Selection on the Metabolic Activities and Drug Susceptibilities of Clostridium perfringens Strains

Fluoroquinolone resistance affects toxin production of Clostridium perfringens strains differently. To investigate the effect of fluoroquinolone resistance selection on global changes in metabolic activities and drug susceptibilities, four C. perfringens strains and their norfloxacin-, ciprofloxacin...

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Bibliographic Details
Published in:International Journal of Microbiology 2014, Vol.2014 (2014), p.184-191
Main Authors: Park, Miseon, Rafii, Fatemeh
Format: Article
Language:English
Subjects:
Analysis
Antibiotics
Antimicrobial agents
Bacteria
Chemical properties
Clostridium
Clostridium perfringens
DNA repair
Dosage and administration
Drug resistance in microorganisms
Drugs
Genes
Health aspects
Identification and classification
Metabolism
Microbiology
Mutation
Natural selection
Nosocomial infections
Phenotype
Physiological aspects
Quinolone antibacterial agents
Quinolones
Staphylococcus infections
Studies
Toxins
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Summary:Fluoroquinolone resistance affects toxin production of Clostridium perfringens strains differently. To investigate the effect of fluoroquinolone resistance selection on global changes in metabolic activities and drug susceptibilities, four C. perfringens strains and their norfloxacin-, ciprofloxacin-, and gatifloxacin-resistant mutants were compared in nearly 2000 assays, using phenotype microarray plates. Variations among mutant strains resulting from resistance selection were observed in all aspects of metabolism. Carbon utilization, pH range, osmotic tolerance, and chemical sensitivity of resistant strains were affected differently in the resistant mutants depending on both the bacterial genotype and the fluoroquinolone to which the bacterium was resistant. The susceptibilities to gentamicin and erythromycin of all resistant mutants except one increased, but some resistant strains were less susceptible to amoxicillin, cefoxitin, ceftriaxone, chloramphenicol, and metronidazole than their wild types. Sensitivity to ethidium bromide decreased in some resistant mutants and increased in others. Microarray analysis of two gatifloxacin-resistant mutants showed changes in metabolic activities that were correlated with altered expression of various genes. Both the chemical structures of fluoroquinolones and the genomic makeup of the wild types influenced the changes found in resistant mutants, which may explain some inconsistent reports of the effects of therapeutic use of fluoroquinolones on clinical isolates of bacteria.
ISSN:1687-918X
1687-9198
DOI:10.1155/2014/456979