The effect of Longan Arillus extract on enhancing oral absorption of bioactive peptides derived from defatted walnut meal hydrolysates

• Published in: Journal of functional foods 2019-06, Vol.57, p.309-316
• Main Authors: Yang, Xiaoyu, Sheng, Jianyong, Chen, Jitang, Peng, Tianhao, Yin, Xiquan, Liu, Wei, Liang, Ming, Wan, Jiangling, Yang, Xiangliang
• Format: Article
• Language: English
• Subjects: Absorption; Bioactive peptides; Caco-2 cell; Defatted walnut meal hydrolysate; Interaction; Longan Arillus
• ISSN: 1756-4646; 2214-9414
• DOI: 10.1016/j.jff.2019.04.018

[Display omitted] •Extract of Longan Arillus was prepared and characterized by high content of polysaccharides.•Mixing with ELA enhanced absorption of anion peptide derived from defatted walnut meal hydrolysates.•Particles formed by ELA and peptides altered endocytosis pathways of peptides in Caco-2 cells.•Enhancing effect of ELA on absorption of peptides depended on their binding style. The present study aims to investigate the effect of Longan Arillus extract (ELA) on oral absorption of bioactive peptides (cationic P1: VEGNLQVLRPR, anionic P2: HNLDTQTESDV) derived from defatted walnut meal hydrolysates (DWMH). Oral co-delivery of ELA with DWMH led to a significantly higher maximum blood concentration of P2 in rats than delivering DWMH alone. Endocytosis pathway of P2 in intestinal epithelia cells was altered by ELA, probably resulting from formation of uniform microparticles by mixing of P2 and ELA. Results of isothermal calorimetry proved strong binding between molecules of P2 and ELA at a molar ratio of 1:1. On the contrary, the absorption of cationic P1 remained unchanged after co-delivery of ELA, probably resulting from the complicated patterns of binding between ELA and P1 molecules. In conclusion, we found the enhancing effect of polysaccharides on absorption of peptides by formation of microparticles, which could be predicted by molecule binding.