Relationship between IL-8 Circulating Levels and TLR2 Hepatic Expression in Women with Morbid Obesity and Nonalcoholic Steatohepatitis

• Published in: International journal of molecular sciences 2020-06, Vol.21 (11), p.4189
• Main Authors: Auguet, Teresa, Bertran, Laia, Binetti, Jessica, Aguilar, Carmen, Martínez, Salomé, Sabench, Fàtima, Lopez-Dupla, Jesús Miguel, Porras, José Antonio, Riesco, David, Del Castillo, Daniel, Richart, Cristóbal
• Format: Article
• Language: English
• Subjects: Adipokines - blood; Adiponectin; Adult; Bariatric Surgery; Biomarkers; Biopsy; Body mass index; Case-Control Studies; Chemokines; Cytokines; Diagnosis; Disease; Fatty liver; Female; Glucose; Humans; IL-1β; Immune system; Inflammation; Insulin resistance; Interleukin 10; Interleukin 8; Interleukin-8 - blood; Kinases; Liver; Liver - metabolism; Liver - pathology; Liver diseases; Middle Aged; Monocyte chemoattractant protein 1; morbid obesity; Non-alcoholic Fatty Liver Disease - blood; Non-alcoholic Fatty Liver Disease - pathology; nonalcoholic fatty liver disease; nonalcoholic steatohepatitis; Obesity; Obesity, Morbid - blood; Obesity, Morbid - surgery; Pathogenesis; Permeability; Plasma levels; Population; Steatosis; TLR2 protein; TLR4 protein; Toll-Like Receptor 2 - genetics; Toll-Like Receptor 2 - metabolism; Toll-Like Receptor 4 - blood; Toll-Like Receptor 4 - genetics; Toll-Like Receptor 4 - metabolism; Toll-like receptors; Tumor necrosis factor-TNF; Tumor necrosis factor-α; Womens health
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms21114189

The progression of nonalcoholic fatty liver disease (NAFLD) to nonalcoholic steatohepatitis (NASH) is linked to systemic inflammation. Currently, two of the aspects that need further investigation are diagnosis and treatment of NASH. In this sense, the aim of this study was to assess the relationship between circulating levels of cytokines, hepatic expression of toll-like receptors (TLRs), and degrees of NAFLD, and to investigate whether these levels could serve as noninvasive biomarkers of NASH. The present study assessed plasma levels of cytokines in 29 normal-weight women and 82 women with morbid obesity (MO) (subclassified: normal liver ( = 29), simple steatosis ( = 32), and NASH ( = 21)). We used enzyme-linked immunosorbent assays (ELISAs) to quantify cytokine and TLR4 levels and RTqPCR to assess TLRs hepatic expression. IL-1β, IL-8, IL-10, TNF-α, tPAI-1, and MCP-1 levels were increased, and adiponectin levels were decreased in women with MO. IL-8 was significantly higher in MO with NASH than in NL. To sum up, high levels of IL-8 were associated with the diagnosis of NASH in a cohort of women with morbid obesity. Moreover, a positive correlation between TLR2 hepatic expression and IL-8 circulating levels was found.