MST4 Kinase Inhibitor Hesperadin Attenuates Autophagy and Behavioral Disorder via the MST4/AKT Pathway in Intracerebral Hemorrhage Mice

Background. The aim of this study was to explore the role of hesperadin in intracerebral hemorrhage (ICH) mice, with the involvement of the mammalian ste20-like kinase 4 (MST4)/AKT signaling pathway. Methods. All mice were divided into four groups: sham group, sham+hesperidin group, ICH group, and I...

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Published in:Behavioural neurology 2020-02, Vol.2020 (2020), p.1-9
Main Authors: Xu, Yang, Chu, Zhaohu, Liu, Hairong, Wang, Qinghua, Hu, Wenjie, Wu, Jinting, Wu, Xiaodong, Lv, Kun
Format: Article
Language:English
Subjects:
Animals
Antigens
Apoptosis - drug effects
Autophagy
Autophagy - drug effects
Behavior disorders
Brain - drug effects
Brain Edema - physiopathology
Cerebral Hemorrhage - metabolism
Cerebral Hemorrhage - physiopathology
Edema
Ethylenediaminetetraacetic acid
Experiments
Hemorrhage
Immunoglobulins
Indoles - metabolism
Indoles - pharmacology
Intracerebral hemorrhage
Kinases
Laboratory animals
Male
Mental illness
Mice
Mice, Inbred C57BL
Neurophysiology
Neuroprotective Agents - pharmacology
Phosphorylation
Protein Serine-Threonine Kinases - antagonists & inhibitors
Protein Serine-Threonine Kinases - metabolism
Proteins
Proto-Oncogene Proteins c-akt
Serine-Threonine Kinase 3
Signal Transduction - drug effects
Statistical analysis
Sulfonamides - metabolism
Sulfonamides - pharmacology
Traumatic brain injury
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Summary:Background. The aim of this study was to explore the role of hesperadin in intracerebral hemorrhage (ICH) mice, with the involvement of the mammalian ste20-like kinase 4 (MST4)/AKT signaling pathway. Methods. All mice were divided into four groups: sham group, sham+hesperidin group, ICH group, and ICH+hesperadin group. The effects of hesperadin were assessed on the basis of brain edema and neurobehavioral function. Furthermore, we observed MST4, AKT, phosphorylation of AKT (pAKT), and microtubule-associated protein light chain 3 (LC3) by western blotting. Protein localization of MST4 and LC3 was determined by immunofluorescence. Results. The expression of MST4 was upregulated at 12 h and 24 h after ICH. Brain edema was significantly decreased and neurological function was improved in the hesperadin treatment group compared to the ICH group (P
ISSN:0953-4180
1875-8584
DOI:10.1155/2020/2476861