CCR6+ group 3 innate lymphoid cells accumulate in inflamed joints in rheumatoid arthritis and produce Th17 cytokines

Recent studies show that innate lymphoid cells (ILCs) contribute to the development of chronic inflammation and autoimmune disease. In this study, we assessed the ILC function in the development of rheumatoid arthritis (RA). In a mouse model of collagen-induced arthritis (CIA), we identified and pur...

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Published in:Arthritis research & therapy 2019-08, Vol.21 (1), p.198-198, Article 198
Main Authors: Takaki-Kuwahara, Ayako, Arinobu, Yojiro, Miyawaki, Kohta, Yamada, Hisakata, Tsuzuki, Hirofumi, Irino, Kensuke, Ayano, Masahiro, Kimoto, Yasutaka, Mitoma, Hiroki, Akahoshi, Mitsuteru, Tsukamoto, Hiroshi, Horiuchi, Takahiko, Niiro, Hiroaki, Akashi, Koichi
Format: Article
Language:English
Subjects:
Anopheles
Arthritis
Care and treatment
CCR6
Cells (Biology)
Cellular biology
Collagen
Cytokines
Diagnosis
Fluorescence
Inflammation
Innate lymphoid cells
Laboratory rats
Lymphoid tissue
Medical research
Messenger RNA
Polymerase chain reaction
Rheumatoid arthritis
Rheumatoid factor
RNA
Rodents
Th17 cytokines
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Summary:Recent studies show that innate lymphoid cells (ILCs) contribute to the development of chronic inflammation and autoimmune disease. In this study, we assessed the ILC function in the development of rheumatoid arthritis (RA). In a mouse model of collagen-induced arthritis (CIA), we identified and purified the ILC subsets in peripheral blood (PB), local lymph nodes (LNs), and joints by fluorescence-activated cell sorting and used quantitative PCR to assess the expression levels of representative cytokines. We also correlated the frequencies of each ILC subset in synovial fluid (SF) with clinical parameters in RA patients. In the CIA model, the proportion of CCR6+ ILC3s to total ILCs in joints with active inflammation significantly increased relative to non-arthritic joints (median 29.6% vs 16.7%, p = 0.035). CCR6+ ILC3s from mice with arthritis expressed significantly higher levels of IL-17A and IL-22 mRNA than did comparable cells from control mice (p 
ISSN:1478-6362
1478-6354
1478-6362
DOI:10.1186/s13075-019-1984-x