Hyperoside alleviates toxicity of β-amyloid via endoplasmic reticulum-mitochondrial calcium signal transduction cascade in APP/PS1 double transgenic Alzheimer's disease mice

• Published in: Redox biology 2023-05, Vol.61, p.102637-102637, Article 102637
• Main Authors: Song, Lin Lin, Qu, Yuan Qing, Tang, Yong Pei, Chen, Xi, Lo, Hang Hong, Qu, Li Qun, Yun, Yun Xiao, Wong, Vincent Kam Wai, Zhang, Rui Long, Wang, Hui Miao, Liu, Meng Han, Zhang, Wei, Zhang, Hui Xia, Chan, Joyce Tsz Wai, Wang, Cai Ren, Wu, Jian Hui, Law, Betty Yuen Kwan
• Format: Article
• Language: English
• Subjects: Alzheimer Disease - metabolism; Alzheimer's disease; Amyloid beta-Peptides - metabolism; Animals; Aβ aggregates; Calcium - metabolism; Calcium signal; Disease Models, Animal; Endoplasmic Reticulum - metabolism; Endoplasmic reticulum-mitochondrial-Ca2; Hyperoside; Mice; Mice, Transgenic; Mitochondrial membrane potential; Neurotoxicity; Research Paper; Ryanodine receptors; Signal Transduction
• ISSN: 2213-2317; 2213-2317
• DOI: 10.1016/j.redox.2023.102637

Alzheimer's disease is a neurodegenerative disorder characterized by a decline in cognitive function. The β-amyloid (Aβ) hypothesis suggests that Aβ peptides can spontaneously aggregate into β-fragment-containing oligomers and protofibrils, and this activation of the amyloid pathway alters Ca2+ signaling in neurons, leading to neurotoxicity and thus apoptosis of neuronal cells. In our study, a blood-brain barrier crossing flavonol glycoside hyperoside was identified with anti-Aβ aggregation, BACE inhibitory, and neuroprotective effect in cellular or APP/PSEN1 double transgenic Alzheimer's disease mice model. While our pharmacokinetic data confirmed that intranasal administration of hyperoside resulted in a higher bio-availability in mice brain, further in vivo studies revealed that it improved motor deficit, spatial memory and learning ability of APP/PSEN1 mice with reducing level of Aβ plaques and GFAP in the cortex and hippocampus. Bioinformatics, computational docking and in vitro assay results suggested that hyperoside bind to Aβ and interacted with ryanodine receptors, then regulated cellular apoptosis via endoplasmic reticulum-mitochondrial calcium (Ca2+) signaling pathway. Consistently, it was confirmed that hyperoside increased Bcl2, decreased Bax and cyto-c protein levels, and ameliorated neuronal cell death in both in vitro and in vivo model. By regulating Aβ-induced cell death via regulation on Ca2+ signaling cascade and mitochondrial membrane potential, our study suggested that hyperoside may work as a potential therapeutic agent or preventive remedy for Alzheimer's disease. [Display omitted] •HYP inhibits Aβ aggregation-induced neurotoxicity via direct binding to Aβ.•HYP targets the endoplasmic reticulum-mitochondrial Ca2+ signal cascade through RyR2.•Nasal administration increases bioavailability of HYP in APP/PS1 mice brain.•HYP attenuates Aβ plaque deposition in the cortex and hippocampus of APP/PS1 mice.•HYP improves learning and cognitive abilities in APP/PS1 mice.