One-Pot Synthesis of Silver Nanoparticles Derived from Aqueous Leaf Extract of Ageratum conyzoides and Their Biological Efficacy

• Published in: Antibiotics (Basel) 2023-04, Vol.12 (4), p.688
• Main Authors: Paramasivam, Deepak, Balasubramanian, Balamuralikrishnan, Suresh, Ramya, Kumaravelu, Jayanthi, Vellingiri, Manon Mani, Liu, Wen-Chao, Meyyazhagan, Arun, Alanazi, Amer M, Rengasamy, Kannan R R, Arumugam, Vijaya Anand
• Format: Article
• Language: English
• Subjects: Acarbose; Ageratum conyzoides; antibacterial; antidiabetic; Antiprotozoal agents; Biological properties; Cancer; Carbohydrates; Diabetes; Drug development; FE-SEM; Herbal medicine; HR-TEM; Hydrogen peroxide; Hyperglycemia; Industrialized nations; Malaria; Medicine; Metabolites; Nanoparticles; Natural resources; Phytochemicals; Plant extracts; Population; Scavenging; Silver; silver nanoparticles; Silver nitrate; Spectroscopy; α-Amylase
• ISSN: 2079-6382; 2079-6382
• DOI: 10.3390/antibiotics12040688

The main objective of the present research work is to assess the biological properties of the aqueous plant extract (ACAE) synthesised silver nanoparticles from the herbal plant , and their biological applications. The silver nanoparticle syntheses from Ageratum conyzoides (Ac-AgNPs) were optimised with different parameters, such as pH (2, 4, 6, 8 and 10) and varied silver nitrate concentration (1 mM and 5 mM). Based on the UV-vis spectroscopy analysis of the synthesised silver nanoparticles, the concentration of 5 mM with the pH at 8 was recorded as the peak reduction at 400 nm; and these conditions were optimized were used for further studies. The results of the FE-SEM analysis recorded the size ranges (~30-90 nm), and irregular spherical and triangular shapes of the AC-AgNPs were captured. The characterization reports of the HR-TEM investigation of AC-AgNPs were also in line with the FE-SEM studies. The antibacterial efficacies of AC-AgNPs have revealed the maximum zone of inhibition against to be within 20 mm. The in vitro antiplasmodial activity of AC-AgNPs is shown to have an effective antiplasmodial property (IC :17.65 μg/mL), whereas AgNO has shown a minimum level of IC : value 68.03 μg/mL, and the Ac-AE showed >100 μg/mL at 24 h of parasitaemia suppression. The α-amylase inhibitory properties of AC-AgNPs have revealed a maximum inhibition similar to the control Acarbose (IC : 10.87 μg/mL). The antioxidant activity of the AC-AgNPs have revealed a better property (87.86% ± 0.56, 85.95% ± 1.02 and 90.11 ± 0.29%) when compared with the Ac-AE and standard in all the three different tests, such as DPPH, FRAP and H O scavenging assay, respectively. The current research work might be a baseline for the future drug expansion process in the area of nano-drug design, and its applications also has a lot of economic viability and is a safer method in synthesising or producing silver nanoparticles.