Immune-Enhancing Effects of Limosilactobacillus fermentum in BALB/c Mice Immunosuppressed by Cyclophosphamide

This study evaluates the immune-enhancing effects of on cyclophosphamide (CP)-induced immunosuppression in BALB/c mice. In vitro, the expressions of pro-inflammatory cytokines and MAPK signaling molecules in Raw264.7 cells were analyzed by ELISA and Western blot analysis. Moreover, cell proliferatio...

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Bibliographic Details
Published in:Nutrients 2023-02, Vol.15 (4), p.1038
Main Authors: Kim, SukJin, Lee, Hwan Hee, Kang, Chang-Ho, Kang, Hyojeung, Cho, Hyosun
Format: Article
Language:English
Subjects:
Animals
Antibodies
Biocompatibility
Cell growth
Cell proliferation
Cyclophosphamide
Cytokines
Cytokines - metabolism
Cytotoxicity
Enzyme-linked immunosorbent assay
Flow cytometry
Food additives
Functional foods & nutraceuticals
Immune system
immune-enhancing effects
Immunosuppression
In vivo methods and tests
Inflammation
Kinases
L. fermentum
Leukocytes
Limosilactobacillus fermentum
MAP kinase
Mice
Mice, Inbred BALB C
Neutrophils
Oral administration
Probiotics
Spleen
Splenocytes
Thymus gland
Tumor Necrosis Factor-alpha - metabolism
Tumor necrosis factor-TNF
Tumor necrosis factor-α
γ-Interferon
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Summary:This study evaluates the immune-enhancing effects of on cyclophosphamide (CP)-induced immunosuppression in BALB/c mice. In vitro, the expressions of pro-inflammatory cytokines and MAPK signaling molecules in Raw264.7 cells were analyzed by ELISA and Western blot analysis. Moreover, cell proliferation, surface receptor expression, and cytotoxicity of NK-92 cells were examined by Cell Counting Kit-8, CytoTox96 assay, and flow cytometry, respectively. To investigate the immune-enhancing effects of selected strains in vivo, these strains were orally administered to BALB/c mice for 2 weeks, and CP was intraperitoneally injected. Then, liver, spleen, and whole blood were isolated from each animal. Administration of single strains or their mixture sustained the spleen weight, the counts of white blood cells compared to non-fed group. Splenocyte proliferation and NK cytotoxicity were significantly increased in all -fed groups. The frequency of B220+ cells was also significantly enhanced in splenocytes isolated from groups. In addition, the production of cytokines (TNF-α, IFN-γ) and antibodies was recovered in splenocyte supernatants isolated from groups. In conclusion, could be a suitable functional food additive for immune-enhancing effect.
ISSN:2072-6643
2072-6643
DOI:10.3390/nu15041038