Hyperspectral Detection and Monitoring of Salt Stress in Pomegranate Cultivars

• Published in: Agronomy (Basel) 2021, Vol.11 (6), p.1038
• Main Authors: Calzone, Antonella, Cotrozzi, Lorenzo, Lorenzini, Giacomo, Nali, Cristina, Pellegrini, Elisa
• Format: Article
• Language: English
• Subjects: Abiotic stress; Agriculture; chlorophyll a fluorescence; Collections; Crop yield; Cultivars; Goodness of fit; high-throughput phenotyping; Least squares method; Leaves; Mathematical models; Morphology; osmotic potential; Oxidative stress; Parameter estimation; partial least discriminant analysis; partial least squares regression; Phenotyping; Physiology; Precision farming; Prediction models; Punica granatum; Regression analysis; Root-mean-square errors; Salinity; Salinity effects; Salinity tolerance; Salt; Salt tolerance; Sensors; Sodium chloride; Spectra; Spectral signatures; Spectrum analysis; Vegetation
• ISSN: 2073-4395; 2073-4395
• DOI: 10.3390/agronomy11061038

Advancements in the ability to detect plant responses to salinity are mandatory to improve crop yield, quality, and management practices. The present study shows the capability of hyperspectral reflectance (400–2400 nm) to rapidly and non-destructively detect and monitor the responses of two pomegranate cultivars (Parfianka, P, and Wonderful, W) under salt treatment (i.e., 200 mL of 100 mM NaCl solution every day) for 35 days. Analyzing spectral signatures from asymptomatic leaves, the two cultivars, as well as salinity conditions were discriminated. Furthermore, using a partial least squares regression approach, we constructed predictive models to concomitantly estimate (goodness-of-fit model, R2: 0.61–0.79; percentage of the root mean square error over the data range, %RMSE: 9–14) from spectra of various physiological leaf parameters commonly investigated in plant/salinity studies. The analyses of spectral signatures enabled the early detection of salt stress (i.e., from 14 days from the beginning of treatment, FBT), even in the absence of visible symptoms, but they did not allow the identification of the different degrees of salt tolerance between cultivars; this cultivar-specific tolerance to salt was instead reported by analyzing variations of leaf parameters estimated from spectra (W was less tolerant than P), which, in turn, allowed the detection of salt stress only at later times of analysis (i.e., slightly from 21 day FBT and, evidently, at the end of treatment). The proposed approach could be used in precision agriculture, high-throughput plant phenotyping, and smart nursery management to enhance crop quality and yield.