Development and evaluation of 16 new microsatellite loci for the rock ptarmigan (Lagopus muta) and cross-species amplification for the willow grouse (L. lagopus)

The genetic markers designed for this study can facilitate future genetic studies on the rock ptarmigan (Lagopus muta). To our knowledge no microsatellite markers have ever been developed specifically for this species before. These new microsatellite markers will be useful for population genetics st...

Full description

Saved in:
Bibliographic Details
Published in:BMC research notes 2018-02, Vol.11 (1), p.147-147, Article 147
Main Authors: Costanzi, Jean-Marc, Bergan, Frode, Sæbø, Mona, Jenkins, Andrew, Steifetten, Øyvind
Format: Article
Language:English
Subjects:
Animals
Birds
Conserved sequence
Feces
Galliformes - genetics
Genetic Loci
Genetic markers
Genetics, Population - methods
Heterozygosity
High-Throughput Nucleotide Sequencing
Laboratories
Lagopus lagopus
Lagopus muta
Microsatellite Repeats - genetics
Microsatellites
Mitochondrial DNA
Next-generation sequencing
Norway
Population genetics
Population studies
Research Note
Software
Sweden
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The genetic markers designed for this study can facilitate future genetic studies on the rock ptarmigan (Lagopus muta). To our knowledge no microsatellite markers have ever been developed specifically for this species before. These new microsatellite markers will be useful for population genetics studies and for future conservation projects. Using Next Generation Sequencing 6252 potential microsatellite sequences were found. Sixteen nonpalindromic tetranucleotide microsatellites and their respective primers were selected. The markers were tested on both the rock ptarmigan and the willow grouse (L. lagopus). The number of alleles varied between 2 and 18 for the rock ptarmigan, and between 3 and 13 for the willow grouse. Expected heterozygosity was in the range 0.1244-0.8692 and 0.1358-0.8722 for the rock ptarmigan and the willow grouse, respectively.
ISSN:1756-0500
1756-0500
DOI:10.1186/s13104-018-3249-1