Blood Supplementation Enhances Bartonella henselae Growth and Molecular Detection of Bacterial DNA in Liquid Culture

Bacteria of the genus , a member of the , are fastidious, Gram-negative, aerobic bacilli that comprise numerous species, subspecies, and genotypes. Bartonella henselae, with a worldwide distribution, infects cats, dogs, horses, humans, and other mammals. Diagnostically, direct detection of Bartonell...

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Bibliographic Details
Published in:Microbiology spectrum 2023-06, Vol.11 (3), p.e0512622-e0512622
Main Authors: Liedig, Chance, Neupane, Pradeep, Lashnits, Erin, Breitschwerdt, Edward B, Maggi, Ricardo G
Format: Article
Language:English
Subjects:
BAPGM
Bartonella culture
Bartonella henselae
bartonellosis
blood supplementation
Clinical Microbiology
diagnostics
Research Article
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Summary:Bacteria of the genus , a member of the , are fastidious, Gram-negative, aerobic bacilli that comprise numerous species, subspecies, and genotypes. Bartonella henselae, with a worldwide distribution, infects cats, dogs, horses, humans, and other mammals. Diagnostically, direct detection of Bartonella henselae in patient blood specimens by culture or molecular methods is required to confirm infection with this bacterium. Enrichment blood culture combined with quantitative PCR (qPCR) or ddPCR enhances the sensitivity of direct detection. The addition of sheep blood to liquid culture media increased the Bartonella henselae DNA concentration compared to controls, additionally improving PCR direct detection sensitivity. This study aims to improve diagnostic detection of Bartonella henselae. Patient samples are combined with enriched bacterial cultures aimed at growing Bartonella henselae for the best possible chance at detection. However, current growth methods could be improved. The DNA extraction method used by most laboratories should also be optimized. Sheep blood was added to increase the growth of Bartonella henselae and multiple DNA extraction methods were to be compared to each other.
ISSN:2165-0497
2165-0497
DOI:10.1128/spectrum.05126-22