Generation of an induced pluripotent stem cell line from a healthy Caucasian male

The effects of genetic mutations on protein function can be studied in a physiologically relevant environment using tissue-specific cells differentiated from patient-derived induced pluripotent stem cells (iPSC). However, it is crucial to use iPSC derived from healthy individuals as control. We gene...

Full description

Saved in:
Bibliographic Details
Published in:Stem cell research 2022-04, Vol.60, p.102717-102717, Article 102717
Main Authors: Martínez-Moreno, Rebecca, Pérez-Serra, Alexandra, Carreras, David, Aran, Begoña, Kuebler, Bernd, Brugada, Ramon, Scornik, Fabiana S., Pérez, Guillermo J., Selga, Elisabet
Format: Article
Language:English
Subjects:
Cell Differentiation
Cell Line
Fibroblasts
Germ Layers
Humans
Induced Pluripotent Stem Cells - metabolism
Male
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The effects of genetic mutations on protein function can be studied in a physiologically relevant environment using tissue-specific cells differentiated from patient-derived induced pluripotent stem cells (iPSC). However, it is crucial to use iPSC derived from healthy individuals as control. We generated an iPS cell line from skin fibroblasts of a healthy Caucasian male by nucleofection of non-integrating episomal vectors. This cell line has normal karyotype, expresses pluripotency surface markers and pluripotency genes, and successfully differentiates into cells of the 3 germ layers. Therefore, it can be used as control for any disease of interest that is modelled using iPSC.
ISSN:1873-5061
1876-7753
DOI:10.1016/j.scr.2022.102717