Development of monoclonal antibodies against oropouche virus and its applicability to immunohistochemical diagnosis

Orthobunyavirus oropouche ense virus (OROV), the causative agent of Oropouche fever, is widely dispersed in Brazil and South America, causing sporadic outbreaks. Due to the similarity of initial clinical symptoms caused by OROV with other arboviruses found in overlapping geographical areas, differen...

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Bibliographic Details
Published in:Virology journal 2024-04, Vol.21 (1), p.81-81, Article 81
Main Authors: Andreolla, Ana Paula, Borges, Alessandra Abel, Nagashima, Seigo, Vaz de Paula, Caroline Busatta, de Noronha, Lucia, Zanchin, Nilson I T, Bordignon, Juliano, Duarte Dos Santos, Claudia Nunes
Format: Article
Language:English
Subjects:
Analysis
Animals
Antibodies, Monoclonal
Antigens
Arbovirus diseases
Brazil - epidemiology
Bunyaviridae Infections - diagnosis
Cloning
Dengue fever
Diagnosis
Differential diagnosis
Dosage and administration
Drug therapy
Epidemiology
Ethylenediaminetetraacetic acid
Health aspects
Immune-histochemistry diagnosis
Immunization
Immunohistochemistry
Indirect immunofluorescent assay
Infection
Infections
Ketamine
Mice
Monoclonal antibodies
Monoclonal antibody
Multiple myeloma
Neglected virus
Nucleocapsids
Oropouche virus
Orthobunyavirus
Penicillin
Polyethylene glycol
Prevalence studies (Epidemiology)
Proteins
RNA polymerase
Serological surveys
Testing
Viral infections
Zika virus
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Description
Summary:Orthobunyavirus oropouche ense virus (OROV), the causative agent of Oropouche fever, is widely dispersed in Brazil and South America, causing sporadic outbreaks. Due to the similarity of initial clinical symptoms caused by OROV with other arboviruses found in overlapping geographical areas, differential diagnosis is challenging. As for most neglected tropical diseases, there is a shortage of reagents for diagnosing and studying OROV pathogenesis. We therefore developed and characterized mouse monoclonal antibodies and, one of them recognizes the OROV nucleocapsid in indirect immunofluorescent (IFA) and immunohistochemistry (IHC) assays. Considering that it is the first monoclonal antibody produced for detecting OROV infections, we believe that it will be useful not only for diagnostic purposes but also for performing serological surveys and epidemiological surveillance on the dispersion and prevalence of OROV in Brazil and South America.
ISSN:1743-422X
1743-422X
DOI:10.1186/s12985-024-02323-z