A Sustainable Biomineralization Approach for the Synthesis of Highly Fluorescent Ultra-Small Pt Nanoclusters

Herein we report the first example of a facile biomineralization process to produce ultra-small-sized highly fluorescent aqueous dispersions of platinum noble metal quantum clusters (Pt-NMQCs) using a multi-stimulus responsive, biomimetic intrinsically disordered protein (IDP), Rec1-resilin. We demo...

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Bibliographic Details
Published in:Biosensors (Basel) 2019-10, Vol.9 (4), p.128
Main Authors: Balu, Rajkamal, Knott, Robert, Elvin, Christopher M, Hill, Anita J, R Choudhury, Namita, Dutta, Naba K
Format: Article
Language:English
Subjects:
Amino acids
Aqueous solutions
Biomimetics
biomineralization
Biosensing Techniques
biosensors
Catalysis
E coli
Evaporation
Fluorescence
Fluorescence spectroscopy
Fluorescent Dyes - chemical synthesis
Fluorescent Dyes - chemistry
fluorescent platinum nanoclusters
Insects
intrinsically disordered protein
Metal Nanoparticles - chemistry
Mineralization
Molecular weight
Nanoclusters
noble metal clusters
Noble metals
Oxidation
Peptides
Photoelectron spectroscopy
Photoelectrons
Photoluminescence
Photons
Platinum
Platinum - chemistry
protein polymer
Proteins
quantum dot
Quantum dots
Reducing agents
small angle x-ray scattering
Spectrometry, Fluorescence
Spectroscopy
Valence
X-ray scattering
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Summary:Herein we report the first example of a facile biomineralization process to produce ultra-small-sized highly fluorescent aqueous dispersions of platinum noble metal quantum clusters (Pt-NMQCs) using a multi-stimulus responsive, biomimetic intrinsically disordered protein (IDP), Rec1-resilin. We demonstrate that Rec1-resilin acts concurrently as the host, reducing agent, and stabilizer of the blue-green fluorescent Pt-NMQCs once they are being formed. The photophysical properties, quantum yield, and fluorescence lifetime measurements of the synthesized Pt-NMQCs were examined using UV-Vis and fluorescence spectroscopy. The oxidation state of the Pt-NMQCs was quantitatively analyzed using X-ray photoelectron spectroscopy. Both a small angle X-ray scattering technique and a modeling approach have been attempted to present a detailed understanding of the structure and conformational dynamics of Rec1-resilin as an IDP during the formation of the Pt-NMQCs. It has been demonstrated that the green fluorescent Pt-NMQCs exhibit a high quantum yield of ~7.0% and a lifetime of ~9.5 ns in aqueous media. The change in photoluminescence properties due to the inter-dot interactions between proximal dots and aggregation of the Pt-NMQCs by evaporation was also measured spectroscopically and discussed.
ISSN:2079-6374
2079-6374
DOI:10.3390/bios9040128