Effect of ephrin-B2 on the expressions of angiopoietin-1 and -2 after focal cerebral ischemia/ reperfusion

Ephrin-B2 has been shown to participate in angiogenesis, but the underlying mechanisms involved remain unclear. In this study, a rat model of local cerebral ischemia was prepared by focal middle cerebral artery occlusion, followed by 24-hour reperfusion. Then, ephrin-B2 protein was administered intr...

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Bibliographic Details
Published in:Neural regeneration research 2016-11, Vol.11 (11), p.1784-1789
Main Authors: Xiao, Hui, Huang, Qing, Wang, Jia-qi, Deng, Qing-qing, Gu, Wen-ping
Format: Article
Language:English
Subjects:
Analysis
Angiogenesis
Cerebral ischemia
Ephrin B
Ischemia
Laboratory animals
Messenger RNA
mRNA
Neovascularization
nerve regeneration
focal cerebral ischemia/reperfusion
ephrin-B2
angiogenesis
angiopoietin-1
angiopoietin-2
neuroprotection
Polyclonal antibodies
Proteins
Reperfusion (Physiology)
Rodents
Signal transduction
Software
Surgical outcomes
免疫荧光染色
实时定量
局灶性脑缺血再灌注
血管内皮细胞
血管新生
血管生成素
逆转录PCR
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Summary:Ephrin-B2 has been shown to participate in angiogenesis, but the underlying mechanisms involved remain unclear. In this study, a rat model of local cerebral ischemia was prepared by focal middle cerebral artery occlusion, followed by 24-hour reperfusion. Then, ephrin-B2 protein was administered intracerebroventricularly for 3 consecutive days via a micro-osmotic pump. Western blot assay and quantitative real-time reverse transcription PCR demonstrated the expression levels of angiopoietin-1 （Ang-1） mRNA and protein in the penumbra cortex of the ephrin-B2 treated group were decreased at day 4 after reperfusion, and increased at day 28, while the expression levels of angiopoietin-2 （Ang-2） were highly up-regulated at all time points tested. Double immunofluorescent staining indicated that Ang-1 and Ang-2 were both expressed in vascular endothelial cells positive for CD31. These findings indicate that ephrin-B2 influences the expressions of Ang-1 and Ang-2 during angiogenesis following transient focal cerebral ischemia.
ISSN:1673-5374
1876-7958
DOI:10.4103/1673-5374.194723