Extensive low-density Plasmodium falciparum reservoir in the island of Príncipe, an isolated malaria pre-elimination setting

•Quantitative polymerase chain reaction unveiled 70% of low-density Plasmodium falciparum infections.•Prevalences were even higher in reactive case detection surveys.•Parasite reservoirs contribute to sporadic cases hindering malaria elimination. The isolated Príncipe is at the malaria pre-eliminati...

Full description

Saved in:
Bibliographic Details
Published in:International journal of infectious diseases 2024-10, Vol.147, p.107220, Article 107220
Main Authors: de Sousa, Taís Nóbrega, Machado, Patricia Carneiro, Lopes, Inês, Das Neves, Edvaldo, Narciso, Alda, Pires, Anastácio, Santos, Adalberto, Trovoada, Maria Jesus, Gil, José Pedro, Lopes, Dinora
Format: Article
Language:English
Subjects:
Elimination
Malaria
Medicin och hälsovetenskap
Parasite reservoir
Plasmodium falciparum
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites cdi_FETCH-LOGICAL-c485t-8f1684d8ab9a750631fad581e551a689fe3b8c7e7017c7b857bd84c9108273853
container_end_page
container_issue
container_start_page 107220
container_title International journal of infectious diseases
container_volume 147
creator de Sousa, Taís Nóbrega
Machado, Patricia Carneiro
Lopes, Inês
Das Neves, Edvaldo
Narciso, Alda
Pires, Anastácio
Santos, Adalberto
Trovoada, Maria Jesus
Gil, José Pedro
Lopes, Dinora
description •Quantitative polymerase chain reaction unveiled 70% of low-density Plasmodium falciparum infections.•Prevalences were even higher in reactive case detection surveys.•Parasite reservoirs contribute to sporadic cases hindering malaria elimination. The isolated Príncipe is at the malaria pre-elimination stage. Autochthonous clinical cases have been reported sporadically on the island, signaling the possibility of a sizable subpatent (i.e., rapid diagnostic test- and microscopy-negative and polymerase chain reaction [PCR]-positive) parasite reservoir. Asymptomatic low-density infections were detected by quantitative PCR (qPCR) targeting Plasmodium falciparum multicopy genes (pfr364 and varATS). Positivity rates were assayed for samples surveyed by active case detection (n = 112) and reactive case detection (n = 221) in 2022. qPCR unveiled 70% of low parasitemia carriers, reaching >90% in reactive case detection. The high P. falciparum prevalence was confirmed by the two high-sensitivity qPCR protocols. Higher positivity rates were observed in the localities where most malaria cases were reported in 2022. Most parasitemias were very low (
doi_str_mv 10.1016/j.ijid.2024.107220
format article
fullrecord <record><control><sourceid>proquest_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_4cd73c89d6b643a0848b96f9d5f0b43b</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S1201971224002911</els_id><doaj_id>oai_doaj_org_article_4cd73c89d6b643a0848b96f9d5f0b43b</doaj_id><sourcerecordid>3099140383</sourcerecordid><originalsourceid>FETCH-LOGICAL-c485t-8f1684d8ab9a750631fad581e551a689fe3b8c7e7017c7b857bd84c9108273853</originalsourceid><addsrcrecordid>eNqdUs1u1DAYjBCI_sALcEA-ciCLHTuxLXFBVSmVKtEDnC3H_lK-JYkX27ulBx6Jp-DF8JKlggsHDpY_jWbG1jdTVc8YXTHKulfrFa7RrxraiALIpqEPqmOmpKp5y9jDMjeU1Vqy5qg6SWlNKRVdpx5XR1wzLaUQx9W3868Z5oQ7IGO4rf1-znfkerRpCh63Exns6HBjYxkjJIi7gJHgTPInIJhGO3sSBnIdf3yfCw9eEjsXPIw2gyeTHW1ESzYRahhxwtlmDDNJkDPON0-qR8U-wdPDfVp9fHv-4exdffX-4vLszVXthGpzrQbWKeGV7bWVLe04G6xvFYO2ZbZTegDeKydBUiad7FUre6-E04yqRnLV8tPqcvH1wa7NJuJk450JFs0vIMQbY2NGN4IRzkvulPZd3wluqRKq192gfTvQXvC-eOnFK93CZtv_5baJwZsD_hn3xyQwze91_4eWtSW_tkRbtC8WbSF-2ULKZsLkYCwJQNgmw6nWTFCueKE2C9XFkFKE4f4hRs2-OmZt9tUx--qYpTpF9Pzgv-0n8PeSPz7_eiFASWqHEE1yCLMDjxFcLqvEf_n_BOqQ2F0</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3099140383</pqid></control><display><type>article</type><title>Extensive low-density Plasmodium falciparum reservoir in the island of Príncipe, an isolated malaria pre-elimination setting</title><source>ScienceDirect Journals</source><creator>de Sousa, Taís Nóbrega ; Machado, Patricia Carneiro ; Lopes, Inês ; Das Neves, Edvaldo ; Narciso, Alda ; Pires, Anastácio ; Santos, Adalberto ; Trovoada, Maria Jesus ; Gil, José Pedro ; Lopes, Dinora</creator><creatorcontrib>de Sousa, Taís Nóbrega ; Machado, Patricia Carneiro ; Lopes, Inês ; Das Neves, Edvaldo ; Narciso, Alda ; Pires, Anastácio ; Santos, Adalberto ; Trovoada, Maria Jesus ; Gil, José Pedro ; Lopes, Dinora</creatorcontrib><description>•Quantitative polymerase chain reaction unveiled 70% of low-density Plasmodium falciparum infections.•Prevalences were even higher in reactive case detection surveys.•Parasite reservoirs contribute to sporadic cases hindering malaria elimination. The isolated Príncipe is at the malaria pre-elimination stage. Autochthonous clinical cases have been reported sporadically on the island, signaling the possibility of a sizable subpatent (i.e., rapid diagnostic test- and microscopy-negative and polymerase chain reaction [PCR]-positive) parasite reservoir. Asymptomatic low-density infections were detected by quantitative PCR (qPCR) targeting Plasmodium falciparum multicopy genes (pfr364 and varATS). Positivity rates were assayed for samples surveyed by active case detection (n = 112) and reactive case detection (n = 221) in 2022. qPCR unveiled 70% of low parasitemia carriers, reaching &gt;90% in reactive case detection. The high P. falciparum prevalence was confirmed by the two high-sensitivity qPCR protocols. Higher positivity rates were observed in the localities where most malaria cases were reported in 2022. Most parasitemias were very low (&lt;2 Pf /µl). These findings suggest that pre-elimination surveillance can benefit from the routine application of highly sensitive tools to unveil otherwise invisible but potentially relevant parasite populations.</description><identifier>ISSN: 1201-9712</identifier><identifier>ISSN: 1878-3511</identifier><identifier>EISSN: 1878-3511</identifier><identifier>DOI: 10.1016/j.ijid.2024.107220</identifier><identifier>PMID: 39197744</identifier><language>eng</language><publisher>Canada: Elsevier Ltd</publisher><subject>Elimination ; Malaria ; Medicin och hälsovetenskap ; Parasite reservoir ; Plasmodium falciparum</subject><ispartof>International journal of infectious diseases, 2024-10, Vol.147, p.107220, Article 107220</ispartof><rights>2024 The Authors</rights><rights>Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c485t-8f1684d8ab9a750631fad581e551a689fe3b8c7e7017c7b857bd84c9108273853</cites><orcidid>0000-0003-0129-7603 ; 0000-0002-6874-9427 ; 0009-0009-0652-3383 ; 0009-0002-9482-4037</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1201971224002911$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>230,314,780,784,885,3549,27924,27925,45780</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39197744$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttp://kipublications.ki.se/Default.aspx?queryparsed=id:159715220$$DView record from Swedish Publication Index$$Hfree_for_read</backlink><backlink>$$Uhttp://kipublications.ki.se/Default.aspx?queryparsed=id:239197744$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><creatorcontrib>de Sousa, Taís Nóbrega</creatorcontrib><creatorcontrib>Machado, Patricia Carneiro</creatorcontrib><creatorcontrib>Lopes, Inês</creatorcontrib><creatorcontrib>Das Neves, Edvaldo</creatorcontrib><creatorcontrib>Narciso, Alda</creatorcontrib><creatorcontrib>Pires, Anastácio</creatorcontrib><creatorcontrib>Santos, Adalberto</creatorcontrib><creatorcontrib>Trovoada, Maria Jesus</creatorcontrib><creatorcontrib>Gil, José Pedro</creatorcontrib><creatorcontrib>Lopes, Dinora</creatorcontrib><title>Extensive low-density Plasmodium falciparum reservoir in the island of Príncipe, an isolated malaria pre-elimination setting</title><title>International journal of infectious diseases</title><addtitle>Int J Infect Dis</addtitle><description>•Quantitative polymerase chain reaction unveiled 70% of low-density Plasmodium falciparum infections.•Prevalences were even higher in reactive case detection surveys.•Parasite reservoirs contribute to sporadic cases hindering malaria elimination. The isolated Príncipe is at the malaria pre-elimination stage. Autochthonous clinical cases have been reported sporadically on the island, signaling the possibility of a sizable subpatent (i.e., rapid diagnostic test- and microscopy-negative and polymerase chain reaction [PCR]-positive) parasite reservoir. Asymptomatic low-density infections were detected by quantitative PCR (qPCR) targeting Plasmodium falciparum multicopy genes (pfr364 and varATS). Positivity rates were assayed for samples surveyed by active case detection (n = 112) and reactive case detection (n = 221) in 2022. qPCR unveiled 70% of low parasitemia carriers, reaching &gt;90% in reactive case detection. The high P. falciparum prevalence was confirmed by the two high-sensitivity qPCR protocols. Higher positivity rates were observed in the localities where most malaria cases were reported in 2022. Most parasitemias were very low (&lt;2 Pf /µl). These findings suggest that pre-elimination surveillance can benefit from the routine application of highly sensitive tools to unveil otherwise invisible but potentially relevant parasite populations.</description><subject>Elimination</subject><subject>Malaria</subject><subject>Medicin och hälsovetenskap</subject><subject>Parasite reservoir</subject><subject>Plasmodium falciparum</subject><issn>1201-9712</issn><issn>1878-3511</issn><issn>1878-3511</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNqdUs1u1DAYjBCI_sALcEA-ciCLHTuxLXFBVSmVKtEDnC3H_lK-JYkX27ulBx6Jp-DF8JKlggsHDpY_jWbG1jdTVc8YXTHKulfrFa7RrxraiALIpqEPqmOmpKp5y9jDMjeU1Vqy5qg6SWlNKRVdpx5XR1wzLaUQx9W3868Z5oQ7IGO4rf1-znfkerRpCh63Exns6HBjYxkjJIi7gJHgTPInIJhGO3sSBnIdf3yfCw9eEjsXPIw2gyeTHW1ESzYRahhxwtlmDDNJkDPON0-qR8U-wdPDfVp9fHv-4exdffX-4vLszVXthGpzrQbWKeGV7bWVLe04G6xvFYO2ZbZTegDeKydBUiad7FUre6-E04yqRnLV8tPqcvH1wa7NJuJk450JFs0vIMQbY2NGN4IRzkvulPZd3wluqRKq192gfTvQXvC-eOnFK93CZtv_5baJwZsD_hn3xyQwze91_4eWtSW_tkRbtC8WbSF-2ULKZsLkYCwJQNgmw6nWTFCueKE2C9XFkFKE4f4hRs2-OmZt9tUx--qYpTpF9Pzgv-0n8PeSPz7_eiFASWqHEE1yCLMDjxFcLqvEf_n_BOqQ2F0</recordid><startdate>20241001</startdate><enddate>20241001</enddate><creator>de Sousa, Taís Nóbrega</creator><creator>Machado, Patricia Carneiro</creator><creator>Lopes, Inês</creator><creator>Das Neves, Edvaldo</creator><creator>Narciso, Alda</creator><creator>Pires, Anastácio</creator><creator>Santos, Adalberto</creator><creator>Trovoada, Maria Jesus</creator><creator>Gil, José Pedro</creator><creator>Lopes, Dinora</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>6I.</scope><scope>AAFTH</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>ADTPV</scope><scope>AOWAS</scope><scope>D8T</scope><scope>ZZAVC</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0003-0129-7603</orcidid><orcidid>https://orcid.org/0000-0002-6874-9427</orcidid><orcidid>https://orcid.org/0009-0009-0652-3383</orcidid><orcidid>https://orcid.org/0009-0002-9482-4037</orcidid></search><sort><creationdate>20241001</creationdate><title>Extensive low-density Plasmodium falciparum reservoir in the island of Príncipe, an isolated malaria pre-elimination setting</title><author>de Sousa, Taís Nóbrega ; Machado, Patricia Carneiro ; Lopes, Inês ; Das Neves, Edvaldo ; Narciso, Alda ; Pires, Anastácio ; Santos, Adalberto ; Trovoada, Maria Jesus ; Gil, José Pedro ; Lopes, Dinora</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c485t-8f1684d8ab9a750631fad581e551a689fe3b8c7e7017c7b857bd84c9108273853</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Elimination</topic><topic>Malaria</topic><topic>Medicin och hälsovetenskap</topic><topic>Parasite reservoir</topic><topic>Plasmodium falciparum</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>de Sousa, Taís Nóbrega</creatorcontrib><creatorcontrib>Machado, Patricia Carneiro</creatorcontrib><creatorcontrib>Lopes, Inês</creatorcontrib><creatorcontrib>Das Neves, Edvaldo</creatorcontrib><creatorcontrib>Narciso, Alda</creatorcontrib><creatorcontrib>Pires, Anastácio</creatorcontrib><creatorcontrib>Santos, Adalberto</creatorcontrib><creatorcontrib>Trovoada, Maria Jesus</creatorcontrib><creatorcontrib>Gil, José Pedro</creatorcontrib><creatorcontrib>Lopes, Dinora</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>SwePub</collection><collection>SwePub Articles</collection><collection>SWEPUB Freely available online</collection><collection>SwePub Articles full text</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>International journal of infectious diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>de Sousa, Taís Nóbrega</au><au>Machado, Patricia Carneiro</au><au>Lopes, Inês</au><au>Das Neves, Edvaldo</au><au>Narciso, Alda</au><au>Pires, Anastácio</au><au>Santos, Adalberto</au><au>Trovoada, Maria Jesus</au><au>Gil, José Pedro</au><au>Lopes, Dinora</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Extensive low-density Plasmodium falciparum reservoir in the island of Príncipe, an isolated malaria pre-elimination setting</atitle><jtitle>International journal of infectious diseases</jtitle><addtitle>Int J Infect Dis</addtitle><date>2024-10-01</date><risdate>2024</risdate><volume>147</volume><spage>107220</spage><pages>107220-</pages><artnum>107220</artnum><issn>1201-9712</issn><issn>1878-3511</issn><eissn>1878-3511</eissn><abstract>•Quantitative polymerase chain reaction unveiled 70% of low-density Plasmodium falciparum infections.•Prevalences were even higher in reactive case detection surveys.•Parasite reservoirs contribute to sporadic cases hindering malaria elimination. The isolated Príncipe is at the malaria pre-elimination stage. Autochthonous clinical cases have been reported sporadically on the island, signaling the possibility of a sizable subpatent (i.e., rapid diagnostic test- and microscopy-negative and polymerase chain reaction [PCR]-positive) parasite reservoir. Asymptomatic low-density infections were detected by quantitative PCR (qPCR) targeting Plasmodium falciparum multicopy genes (pfr364 and varATS). Positivity rates were assayed for samples surveyed by active case detection (n = 112) and reactive case detection (n = 221) in 2022. qPCR unveiled 70% of low parasitemia carriers, reaching &gt;90% in reactive case detection. The high P. falciparum prevalence was confirmed by the two high-sensitivity qPCR protocols. Higher positivity rates were observed in the localities where most malaria cases were reported in 2022. Most parasitemias were very low (&lt;2 Pf /µl). These findings suggest that pre-elimination surveillance can benefit from the routine application of highly sensitive tools to unveil otherwise invisible but potentially relevant parasite populations.</abstract><cop>Canada</cop><pub>Elsevier Ltd</pub><pmid>39197744</pmid><doi>10.1016/j.ijid.2024.107220</doi><orcidid>https://orcid.org/0000-0003-0129-7603</orcidid><orcidid>https://orcid.org/0000-0002-6874-9427</orcidid><orcidid>https://orcid.org/0009-0009-0652-3383</orcidid><orcidid>https://orcid.org/0009-0002-9482-4037</orcidid><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1201-9712
ispartof International journal of infectious diseases, 2024-10, Vol.147, p.107220, Article 107220
issn 1201-9712
1878-3511
1878-3511
language eng
recordid cdi_doaj_primary_oai_doaj_org_article_4cd73c89d6b643a0848b96f9d5f0b43b
source ScienceDirect Journals
subjects Elimination
Malaria
Medicin och hälsovetenskap
Parasite reservoir
Plasmodium falciparum
title Extensive low-density Plasmodium falciparum reservoir in the island of Príncipe, an isolated malaria pre-elimination setting
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-20T20%3A29%3A00IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Extensive%20low-density%20Plasmodium%20falciparum%20reservoir%20in%20the%20island%20of%20Pr%C3%ADncipe,%20an%20isolated%20malaria%20pre-elimination%20setting&rft.jtitle=International%20journal%20of%20infectious%20diseases&rft.au=de%20Sousa,%20Ta%C3%ADs%20N%C3%B3brega&rft.date=2024-10-01&rft.volume=147&rft.spage=107220&rft.pages=107220-&rft.artnum=107220&rft.issn=1201-9712&rft.eissn=1878-3511&rft_id=info:doi/10.1016/j.ijid.2024.107220&rft_dat=%3Cproquest_doaj_%3E3099140383%3C/proquest_doaj_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c485t-8f1684d8ab9a750631fad581e551a689fe3b8c7e7017c7b857bd84c9108273853%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=3099140383&rft_id=info:pmid/39197744&rfr_iscdi=true