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Short antimicrobial peptidomimetic SAMP-5 effective against multidrug-resistant gram-negative bacteria
SAMP-5 is a short histidine-derived antimicrobial peptidomimetic with pendant dialkylated tail. In this study, we evaluated the potential of SAMP-5 as an antimicrobial agent to combat multidrug-resistant gram-negative bacteria. SAMP-5 showed potent antimicrobial activity (minimum inhibitory concentr...
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| Published in: | Journal of analytical science and technology 2021-07, Vol.12 (1), p.1-6, Article 29 |
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| creator | Kim, Eun Young Han, So Hee Kim, Jong Min Kim, Seon-Myung Shin, Song Yub |
| description | SAMP-5 is a short histidine-derived antimicrobial peptidomimetic with pendant dialkylated tail. In this study, we evaluated the potential of SAMP-5 as an antimicrobial agent to combat multidrug-resistant gram-negative bacteria. SAMP-5 showed potent antimicrobial activity (minimum inhibitory concentration 16-64 μg/ml) comparable to melittin against multidrug-resistant
Escherichia coli
(MDREC) and multidrug-resistant (MDRPA). SAMP-5 displayed no cytotoxicity against three mammalian cells such as mouse macrophage RAW264.7, mouse embryonic fibroblast NIH-3T3, and human bone marrow SH-SY5Y cells at the concentration of 128 μg/ml. SAMP-5 showed resistance to proteolytic degradation with pepsin, trypsin, α-chymotrypsin, and proteinase K. Importantly, unlike ciprofloxacin, no antibiotic resistance against SAMP-5 arose for
Pseudomonas aeruginosa
during 7 days of serial passage at 0.5 × MIC. Moreover, SAMP-5 showed synergy or additive effects against MDRPA and MDREC, when it combined with chloramphenicol, ciprofloxacin, and oxacillin. Collectively, our results suggested that SAMP-5 is a promising alternative and adjuvant to treat infections caused by multidrug-resistant gram-negative bacteria. |
| doi_str_mv | 10.1186/s40543-021-00281-7 |
| format | article |
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Escherichia coli
(MDREC) and multidrug-resistant (MDRPA). SAMP-5 displayed no cytotoxicity against three mammalian cells such as mouse macrophage RAW264.7, mouse embryonic fibroblast NIH-3T3, and human bone marrow SH-SY5Y cells at the concentration of 128 μg/ml. SAMP-5 showed resistance to proteolytic degradation with pepsin, trypsin, α-chymotrypsin, and proteinase K. Importantly, unlike ciprofloxacin, no antibiotic resistance against SAMP-5 arose for
Pseudomonas aeruginosa
during 7 days of serial passage at 0.5 × MIC. Moreover, SAMP-5 showed synergy or additive effects against MDRPA and MDREC, when it combined with chloramphenicol, ciprofloxacin, and oxacillin. Collectively, our results suggested that SAMP-5 is a promising alternative and adjuvant to treat infections caused by multidrug-resistant gram-negative bacteria.</description><identifier>ISSN: 2093-3371</identifier><identifier>ISSN: 2093-3134</identifier><identifier>EISSN: 2093-3371</identifier><identifier>DOI: 10.1186/s40543-021-00281-7</identifier><language>eng</language><publisher>Singapore: Springer Singapore</publisher><subject>Analytical Chemistry ; Antibiotic resistance ; Antibiotics ; Antiinfectives and antibacterials ; Antimicrobial activity ; Antimicrobial agents ; Antimicrobial resistance ; Bacteria ; Biodegradation ; Bone marrow ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; Chloramphenicol ; Chloromycetin ; Chymotrypsin ; Ciprofloxacin ; Combination therapy ; Cytotoxicity ; E coli ; Embryo fibroblasts ; Endopeptidase K ; Gram-negative bacteria ; Histidine ; Macrophages ; Mammalian cells ; Minimum inhibitory concentration ; Monitoring/Environmental Analysis ; Multidrug resistance ; Multidrug-resistant gram-negative bacteria ; Oxacillin ; Pepsin ; Proteinase ; Proteolysis ; Proteolytic stability ; Pseudomonas aeruginosa ; Research Article ; Toxicity ; Trypsin</subject><ispartof>Journal of analytical science and technology, 2021-07, Vol.12 (1), p.1-6, Article 29</ispartof><rights>The Author(s) 2021</rights><rights>The Author(s) 2021. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c530t-1ebb3f2c012093ce0b24a384ce18c4be8928b5464f2acfcfe43a0857754c913a3</citedby><cites>FETCH-LOGICAL-c530t-1ebb3f2c012093ce0b24a384ce18c4be8928b5464f2acfcfe43a0857754c913a3</cites><orcidid>0000-0002-3030-7973</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2551418983/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2551418983?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,25731,27901,27902,36989,44566,74869</link.rule.ids></links><search><creatorcontrib>Kim, Eun Young</creatorcontrib><creatorcontrib>Han, So Hee</creatorcontrib><creatorcontrib>Kim, Jong Min</creatorcontrib><creatorcontrib>Kim, Seon-Myung</creatorcontrib><creatorcontrib>Shin, Song Yub</creatorcontrib><title>Short antimicrobial peptidomimetic SAMP-5 effective against multidrug-resistant gram-negative bacteria</title><title>Journal of analytical science and technology</title><addtitle>J Anal Sci Technol</addtitle><description>SAMP-5 is a short histidine-derived antimicrobial peptidomimetic with pendant dialkylated tail. In this study, we evaluated the potential of SAMP-5 as an antimicrobial agent to combat multidrug-resistant gram-negative bacteria. SAMP-5 showed potent antimicrobial activity (minimum inhibitory concentration 16-64 μg/ml) comparable to melittin against multidrug-resistant
Escherichia coli
(MDREC) and multidrug-resistant (MDRPA). SAMP-5 displayed no cytotoxicity against three mammalian cells such as mouse macrophage RAW264.7, mouse embryonic fibroblast NIH-3T3, and human bone marrow SH-SY5Y cells at the concentration of 128 μg/ml. SAMP-5 showed resistance to proteolytic degradation with pepsin, trypsin, α-chymotrypsin, and proteinase K. Importantly, unlike ciprofloxacin, no antibiotic resistance against SAMP-5 arose for
Pseudomonas aeruginosa
during 7 days of serial passage at 0.5 × MIC. Moreover, SAMP-5 showed synergy or additive effects against MDRPA and MDREC, when it combined with chloramphenicol, ciprofloxacin, and oxacillin. Collectively, our results suggested that SAMP-5 is a promising alternative and adjuvant to treat infections caused by multidrug-resistant gram-negative bacteria.</description><subject>Analytical Chemistry</subject><subject>Antibiotic resistance</subject><subject>Antibiotics</subject><subject>Antiinfectives and antibacterials</subject><subject>Antimicrobial activity</subject><subject>Antimicrobial agents</subject><subject>Antimicrobial resistance</subject><subject>Bacteria</subject><subject>Biodegradation</subject><subject>Bone marrow</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Chloramphenicol</subject><subject>Chloromycetin</subject><subject>Chymotrypsin</subject><subject>Ciprofloxacin</subject><subject>Combination therapy</subject><subject>Cytotoxicity</subject><subject>E coli</subject><subject>Embryo fibroblasts</subject><subject>Endopeptidase K</subject><subject>Gram-negative bacteria</subject><subject>Histidine</subject><subject>Macrophages</subject><subject>Mammalian cells</subject><subject>Minimum inhibitory concentration</subject><subject>Monitoring/Environmental Analysis</subject><subject>Multidrug resistance</subject><subject>Multidrug-resistant gram-negative bacteria</subject><subject>Oxacillin</subject><subject>Pepsin</subject><subject>Proteinase</subject><subject>Proteolysis</subject><subject>Proteolytic stability</subject><subject>Pseudomonas aeruginosa</subject><subject>Research 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Yub</creator><general>Springer Singapore</general><general>Springer Nature 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antimicrobial peptidomimetic SAMP-5 effective against multidrug-resistant gram-negative bacteria</title><author>Kim, Eun Young ; Han, So Hee ; Kim, Jong Min ; Kim, Seon-Myung ; Shin, Song Yub</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c530t-1ebb3f2c012093ce0b24a384ce18c4be8928b5464f2acfcfe43a0857754c913a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Analytical Chemistry</topic><topic>Antibiotic resistance</topic><topic>Antibiotics</topic><topic>Antiinfectives and antibacterials</topic><topic>Antimicrobial activity</topic><topic>Antimicrobial agents</topic><topic>Antimicrobial resistance</topic><topic>Bacteria</topic><topic>Biodegradation</topic><topic>Bone marrow</topic><topic>Characterization and Evaluation of Materials</topic><topic>Chemistry</topic><topic>Chemistry and Materials 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Seon-Myung</au><au>Shin, Song Yub</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Short antimicrobial peptidomimetic SAMP-5 effective against multidrug-resistant gram-negative bacteria</atitle><jtitle>Journal of analytical science and technology</jtitle><stitle>J Anal Sci Technol</stitle><date>2021-07-14</date><risdate>2021</risdate><volume>12</volume><issue>1</issue><spage>1</spage><epage>6</epage><pages>1-6</pages><artnum>29</artnum><issn>2093-3371</issn><issn>2093-3134</issn><eissn>2093-3371</eissn><abstract>SAMP-5 is a short histidine-derived antimicrobial peptidomimetic with pendant dialkylated tail. In this study, we evaluated the potential of SAMP-5 as an antimicrobial agent to combat multidrug-resistant gram-negative bacteria. SAMP-5 showed potent antimicrobial activity (minimum inhibitory concentration 16-64 μg/ml) comparable to melittin against multidrug-resistant
Escherichia coli
(MDREC) and multidrug-resistant (MDRPA). SAMP-5 displayed no cytotoxicity against three mammalian cells such as mouse macrophage RAW264.7, mouse embryonic fibroblast NIH-3T3, and human bone marrow SH-SY5Y cells at the concentration of 128 μg/ml. SAMP-5 showed resistance to proteolytic degradation with pepsin, trypsin, α-chymotrypsin, and proteinase K. Importantly, unlike ciprofloxacin, no antibiotic resistance against SAMP-5 arose for
Pseudomonas aeruginosa
during 7 days of serial passage at 0.5 × MIC. Moreover, SAMP-5 showed synergy or additive effects against MDRPA and MDREC, when it combined with chloramphenicol, ciprofloxacin, and oxacillin. Collectively, our results suggested that SAMP-5 is a promising alternative and adjuvant to treat infections caused by multidrug-resistant gram-negative bacteria.</abstract><cop>Singapore</cop><pub>Springer Singapore</pub><doi>10.1186/s40543-021-00281-7</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0002-3030-7973</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Analytical Chemistry Antibiotic resistance Antibiotics Antiinfectives and antibacterials Antimicrobial activity Antimicrobial agents Antimicrobial resistance Bacteria Biodegradation Bone marrow Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Chloramphenicol Chloromycetin Chymotrypsin Ciprofloxacin Combination therapy Cytotoxicity E coli Embryo fibroblasts Endopeptidase K Gram-negative bacteria Histidine Macrophages Mammalian cells Minimum inhibitory concentration Monitoring/Environmental Analysis Multidrug resistance Multidrug-resistant gram-negative bacteria Oxacillin Pepsin Proteinase Proteolysis Proteolytic stability Pseudomonas aeruginosa Research Article Toxicity Trypsin |
| title | Short antimicrobial peptidomimetic SAMP-5 effective against multidrug-resistant gram-negative bacteria |
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