First Detection and Genome Characterization of a New RNA Virus, Hibiscus Betacarmovirus, and a New DNA Virus, Hibiscus Soymovirus, Naturally Infecting Hibiscus spp. in Hawaii

Hibiscus ( spp., family Malvaceae) leaves exhibiting symptoms of mosaic, ringspot, and chlorotic spots were collected in 2020 on Oahu, HI. High-throughput sequencing analysis was conducted on ribosomal RNA-depleted composite RNA samples extracted from symptomatic leaves. About 77 million paired-end...

Full description

Saved in:
Bibliographic Details
Published in:Viruses 2022-12, Vol.15 (1), p.90
Main Authors: Wang, Xupeng, Larrea-Sarmiento, Adriana E, Olmedo-Velarde, Alejandro, Kong, Alexandra, Borth, Wayne, Suzuki, Jon Y, Wall, Marisa M, Melzer, Michael J, Hu, John
Format: Article
Language:English
Subjects:
Amino acids
betacarmovirus
Caulimoviridae
Cloning
Communication
DNA Viruses
Flowers & plants
Genomes
Hawaii
Hibiscus
high-throughput sequencing
Leaves
New species
Next-generation sequencing
Nucleotide sequence
Phylogenetics
Proteins
Quality control
RNA polymerase
RNA viruses
RNA Viruses - genetics
rRNA
Sequence analysis
soymovirus
Viral infections
Viruses
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Hibiscus ( spp., family Malvaceae) leaves exhibiting symptoms of mosaic, ringspot, and chlorotic spots were collected in 2020 on Oahu, HI. High-throughput sequencing analysis was conducted on ribosomal RNA-depleted composite RNA samples extracted from symptomatic leaves. About 77 million paired-end reads and 161,970 contigs were generated after quality control, trimming, and de novo assembly. Contig annotation with BLASTX/BLASTN searches revealed a sequence (contig 1) resembling the RNA virus, hibiscus chlorotic ringspot virus (genus ), and one (contig 2) resembling the DNA virus, peanut chlorotic streak virus (genus ). Further bioinformatic analyses of the complete viral genome sequences indicated that these viruses, with proposed names of hibiscus betacarmovirus and hibiscus soymovirus, putatively represent new species in the genera and , respectively. RT-PCR using specific primers, designed based on the retrieved contigs, coupled with Sanger sequencing, further confirmed the presence of these viruses. An additional 54 hibiscus leaf samples from other locations on Oahu were examined to determine the incidence and distribution of these viruses.
ISSN:1999-4915
1999-4915
DOI:10.3390/v15010090