Viral detection and identification in 20 min by rapid single-particle fluorescence in-situ hybridization of viral RNA

The increasing risk from viral outbreaks such as the ongoing COVID-19 pandemic exacerbates the need for rapid, affordable and sensitive methods for virus detection, identification and quantification; however, existing methods for detecting virus particles in biological samples usually depend on mult...

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Bibliographic Details
Published in:Scientific reports 2021-10, Vol.11 (1), p.19579-19579, Article 19579
Main Authors: Hepp, Christof, Shiaelis, Nicolas, Robb, Nicole C., Vaughan, Alison, Matthews, Philippa C., Stoesser, Nicole, Crook, Derrick, Kapanidis, Achillefs N.
Format: Article
Language:English
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Avian infectious bronchitis
Biological samples
Bronchitis
COVID-19
Fluorescence
Fluorescence in situ hybridization
Genomes
Humanities and Social Sciences
Hybridization
In Situ Hybridization, Fluorescence - methods
Influenza
multidisciplinary
Pandemics
Public health
RNA, Viral - isolation & purification
Science
Science (multidisciplinary)
Severe acute respiratory syndrome coronavirus 2
Viruses
Viruses - genetics
Viruses - isolation & purification
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Summary:The increasing risk from viral outbreaks such as the ongoing COVID-19 pandemic exacerbates the need for rapid, affordable and sensitive methods for virus detection, identification and quantification; however, existing methods for detecting virus particles in biological samples usually depend on multistep protocols that take considerable time to yield a result. Here, we introduce a rapid fluorescence in situ hybridization (FISH) protocol capable of detecting influenza virus, avian infectious bronchitis virus and SARS-CoV-2 specifically and quantitatively in approximately 20 min, in virus cultures, combined nasal and throat swabs with added virus and likely patient samples without previous purification. This fast and facile workflow can be adapted both as a lab technique and a future diagnostic tool in enveloped viruses with an accessible genome.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-021-98972-z