Interferência do acido ascórbico na determinação de açúcares redutores pelo método de Lane e Eynon Ascorbic acid interference in the determination of reducing and total sugars by Lane and Eynon method

Two studies, both set up as completely randomized design, in a 5x5 and 7x5 factorial schemes, evaluated the interference of 5 and 7 ascorbic acid concentrations and 5 glucose or 5 sucrose concentrations, respectively, on the determination of total and reducing sugars by Lane and Eynon method. The as...

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Bibliographic Details
Published in:Química nova 2010-01, Vol.33 (4), p.805-809
Main Authors: José Torquato de Queiroz Tavares, Ricardo Luis Cardoso, João Albany Costa, Francisco de Souza Fadigas, Antônio Augusto Fonseca
Format: Article
Language:English
Subjects:
Fehling solution
reducing power
vitamin C
Online Access:Get full text
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Summary:Two studies, both set up as completely randomized design, in a 5x5 and 7x5 factorial schemes, evaluated the interference of 5 and 7 ascorbic acid concentrations and 5 glucose or 5 sucrose concentrations, respectively, on the determination of total and reducing sugars by Lane and Eynon method. The ascorbic acid reducing power (AARP) over the Fehling liquor interfered in the results of total and reducing sugars. On average the AARP was equivalent to 74.83 and 69.71% of the reducing power of glucose and of hydrolyzed sucrose, respectively. The ascorbic acid was stable in all study conditions.
ISSN:0100-4042
1678-7064
DOI:10.1590/S0100-40422010000400008