Overexpression of Arabidopsis P3B increases heat and low temperature stress tolerance in transgenic sweetpotato

• Published in: BMC plant biology 2017-08, Vol.17 (1), p.139-139, Article 139
• Main Authors: Ji, Chang Yoon, Jin, Rong, Xu, Zhen, Kim, Ho Soo, Lee, Chan-Ju, Kang, Le, Kim, So-Eun, Lee, Hyeong-Un, Lee, Joon Seol, Kang, Chang Ho, Chi, Yong Hun, Lee, Sang Yeol, Xie, Yiping, Li, Hongmin, Ma, Daifu, Kwak, Sang-Soo
• Format: Article
• Language: English
• Subjects: Abiotic stress; Acclimatization - genetics; Acidic ribosomal P-proteins; Agricultural land; Antioxidants; Arabidopsis - genetics; Arabidopsis - metabolism; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Catalase; Chaperones; Cold; Cold Temperature; Cold tolerance; Cultivars; Cultivation; Deoxyribonucleic acid; DNA; Environmental conditions; Enzymes; Gene Expression Regulation, Plant; Genes; Genetic aspects; Genetic engineering; Heat; Heat stress; Heat tolerance; Hydrogen peroxide; Hydrogen storage; Ipomoea batatas - genetics; Ipomoea batatas - growth & development; Ipomoea batatas - physiology; Kinases; Low temperature; Low temperature stress; Membrane permeability; Peroxidase; Phosphorylation; Photosynthesis; Plants, Genetically Modified - genetics; Polyamines; Potatoes; Protein chaperone; Protein synthesis; Proteins; Ribonucleic acid; Ribosomal Proteins - genetics; Ribosomal Proteins - metabolism; RNA; Rodents; Roots; Stress (Physiology); Stress, Physiological - genetics; Stresses; Sustainable agriculture; Sweet potatoes; Sweetpotato; Temperature effects; Temperature tolerance; Thermotolerance - genetics; Transcription; Transgenic plants
• ISSN: 1471-2229; 1471-2229
• DOI: 10.1186/s12870-017-1087-2

Sweetpotato (Ipomoea batatas [L.] Lam) is suitable for growth on marginal lands due to its abiotic stress tolerance. However, severe environmental conditions including low temperature pose a serious threat to the productivity and expanded cultivation of this crop. In this study, we aimed to develop sweetpotato plants with enhanced tolerance to temperature stress. P3 proteins are plant-specific ribosomal P-proteins that act as both protein and RNA chaperones to increase heat and cold stress tolerance in Arabidopsis. Here, we generated transgenic sweetpotato plants expressing the Arabidopsis ribosomal P3 (AtP3B) gene under the control of the CaMV 35S promoter (referred to as OP plants). Three OP lines (OP1, OP30, and OP32) were selected based on AtP3B transcript levels. The OP plants displayed greater heat tolerance and higher photosynthesis efficiency than wild type (WT) plants. The OP plants also exhibited enhanced low temperature tolerance, with higher photosynthesis efficiency and less membrane permeability than WT plants. In addition, OP plants had lower levels of hydrogen peroxide and higher activities of antioxidant enzymes such as peroxidase and catalase than WT plants under low temperature stress. The yields of tuberous roots and aerial parts of plants did not significantly differ between OP and WT plants under field cultivation. However, the tuberous roots of OP transgenic sweetpotato showed improved storage ability under low temperature conditions. The OP plants developed in this study exhibited increased tolerance to temperature stress and enhanced storage ability under low temperature compared to WT plants, suggesting that they could be used to enhance sustainable agriculture on marginal lands.