Comparative evaluation of feeding effects of A1 and A2 cow milk derived casein hydrolysates in diabetic model of rats

• Published in: Journal of functional foods 2020-12, Vol.75, p.104272, Article 104272
• Main Authors: Thakur, Neha, Chauhan, Geeta, Mishra, B.P., Mendiratta, S.K., Pattanaik, A.K., Singh, Thakur Uttam, Karikalan, M., Meshram, Somesh Kumar, Garg, Lalita
• Format: Article
• Language: English
• Subjects: A1/A2 milk; Casein hydrolysates; Type 1 diabetes; β-Casein variants
• ISSN: 1756-4646; 2214-9414
• DOI: 10.1016/j.jff.2020.104272

[Display omitted] •The study was designed with Type-1 diabetes as the backdrop. During the trial period of 60 days, none of the rats became pre-diabetic due to respective hydrolysate feeding.•Anti-diabetic potential of indigenous cow milk (A2) derived casein hydrolysates was not indicated as it failed to offer resistance to diabetes induction using streptozotocin.•Feeding of A1 or A2 cow milk derived casein hydrolystaes did not cause any deteriorative effect on the blood biochemical profile and histopathology of major organs like heart, liver and kidney. The present study was designed to test diabetogenic activity of A1 and A2 casein hydrolysates and other related pathological conditions, in murine species. 6 weeks old adult male wistar rats were selected and first divided into 3 groups (n = 12) as Control, A1CH and A2CH were fed with respective A1 and A2 casein hydrolysate diets for 50 days. On 51st day, each group was divided into 2 sub groups (n = 6) and diabetes was induced in one subgroup of each group using Streptozotocin, which resulted in 6 sub groups. The fasting blood glucose level and other biochemical, diabetic specific parameters and histopathological findings post sacrifice, did not differ significantly (p > 0.05) among control, A1CH and A2CH groups. Whereas, the diabetic rats showed significant (p