Dual function of SF3B2 on chromatin and RNA to regulate transcription in head and neck squamous cell carcinoma

RNA is spliced concomitantly with transcription and the process is organized by RNA splicing factors, transcriptional regulators, and chromatin regulators. RNA is spliced in close proximity to transcription machinery. Hence, some RNA splicing factors may play a role in transcription. Here, we show t...

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Bibliographic Details
Published in:Cell & bioscience 2022-06, Vol.12 (1), p.92-92, Article 92
Main Authors: Kitamura, Koji, Suzuki, Hidefumi, Abe, Ryota, Inohara, Hidenori, Kaneda, Yasufumi, Takahashi, Hidehisa, Nimura, Keisuke
Format: Article
Language:English
Subjects:
Binding sites
Cell growth
Chromatin
Development and progression
DNA
DNA-directed RNA polymerase
Electron transport
Gene expression
Genes
Genetic transcription
Head & neck cancer
Head and neck carcinoma
Leukemia
Medical prognosis
Messenger RNA
Mitochondria
Mutation
Prognosis
Prostate cancer
Proteins
Regulatory sequences
RNA polymerase
Scientific equipment and supplies industry
Splicing factors
Squamous cell carcinoma
Transcription factors
Transcription termination
Tumors
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Summary:RNA is spliced concomitantly with transcription and the process is organized by RNA splicing factors, transcriptional regulators, and chromatin regulators. RNA is spliced in close proximity to transcription machinery. Hence, some RNA splicing factors may play a role in transcription. Here, we show that the splicing factor SF3B2 binds to gene regulatory elements and mRNA to modulate transcription and RNA stability in head and neck squamous cell carcinoma cells. High SF3B2 expression leads to poor prognosis in patients with head and neck squamous cell carcinoma and to progression of tumor growth in mice. SF3B2 promotes tumor growth, owing to its involvement in activation of gene expression associated with mitochondrial electron transport and transcription regulatory region DNA binding. SF3B2 is enriched around the promoter element on chromatin and the transcription termination site on RNA. SF3B2 is involved in the regulation of RNA stability. According to the SF3B2-binding profile, SF3B2 regulates RNA polymerase II activity, in addition to regulating RNA splicing. Mechanistically, SF3B2 promotes the binding of structural maintenance of chromosomes 1A and CCCTC-binding factor (CTCF) to the SF3B2-binding genomic regions. SF3B2 also modulates CTCF transcriptional activity. Our findings indicate that SF3B2 has a dual function in both transcription and RNA stability, leading to head and neck squamous cell carcinoma progression.
ISSN:2045-3701
2045-3701
DOI:10.1186/s13578-022-00812-8