Genetic Dissection of Maize Embryonic Callus Regenerative Capacity Using Multi-Locus Genome-Wide Association Studies

The regenerative capacity of the embryonic callus, a complex quantitative trait, is one of the main limiting factors for maize transformation. This trait was decomposed into five traits, namely, green callus rate (GCR), callus differentiating rate (CDR), callus plantlet number (CPN), callus rooting...

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Published in:Frontiers in plant science 2018-04, Vol.9, p.561-561
Main Authors: Ma, Langlang, Liu, Min, Yan, Yuanyuan, Qing, Chunyan, Zhang, Xiaoling, Zhang, Yanling, Long, Yun, Wang, Lei, Pan, Lang, Zou, Chaoying, Li, Zhaoling, Wang, Yanli, Peng, Huanwei, Pan, Guangtang, Jiang, Zhou, Shen, Yaou
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Language:English
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candidate gene
embryonic callus
maize
multi-locus GWAS
Plant Science
regenerative capacity
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creator Ma, Langlang
Liu, Min
Yan, Yuanyuan
Qing, Chunyan
Zhang, Xiaoling
Zhang, Yanling
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Zou, Chaoying
Li, Zhaoling
Wang, Yanli
Peng, Huanwei
Pan, Guangtang
Jiang, Zhou
Shen, Yaou
description The regenerative capacity of the embryonic callus, a complex quantitative trait, is one of the main limiting factors for maize transformation. This trait was decomposed into five traits, namely, green callus rate (GCR), callus differentiating rate (CDR), callus plantlet number (CPN), callus rooting rate (CRR), and callus browning rate (CBR). To dissect the genetic foundation of maize transformation, in this study multi-locus genome-wide association studies (GWAS) for the five traits were performed in a population of 144 inbred lines genotyped with 43,427 SNPs. Using the phenotypic values in three environments and best linear unbiased prediction (BLUP) values, as a result, a total of 127, 56, 160, and 130 significant quantitative trait nucleotides (QTNs) were identified by mrMLM, FASTmrEMMA, ISIS EM-BLASSO, and pLARmEB, respectively. Of these QTNs, 63 QTNs were commonly detected, including 15 across multiple environments and 58 across multiple methods. Allele distribution analysis showed that the proportion of superior alleles for 36 QTNs was
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This trait was decomposed into five traits, namely, green callus rate (GCR), callus differentiating rate (CDR), callus plantlet number (CPN), callus rooting rate (CRR), and callus browning rate (CBR). To dissect the genetic foundation of maize transformation, in this study multi-locus genome-wide association studies (GWAS) for the five traits were performed in a population of 144 inbred lines genotyped with 43,427 SNPs. Using the phenotypic values in three environments and best linear unbiased prediction (BLUP) values, as a result, a total of 127, 56, 160, and 130 significant quantitative trait nucleotides (QTNs) were identified by mrMLM, FASTmrEMMA, ISIS EM-BLASSO, and pLARmEB, respectively. Of these QTNs, 63 QTNs were commonly detected, including 15 across multiple environments and 58 across multiple methods. Allele distribution analysis showed that the proportion of superior alleles for 36 QTNs was &lt;50% in 31 elite inbred lines. 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subjects candidate gene
embryonic callus
maize
multi-locus GWAS
Plant Science
regenerative capacity
title Genetic Dissection of Maize Embryonic Callus Regenerative Capacity Using Multi-Locus Genome-Wide Association Studies
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