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Identification of antibiotic resistance genes in Escherichia coli from subclinical mastitis milk in dairy cows and goats, East Java Province
Antibiotics are still used to treat mastitis in dairy cows in Indonesia. This study aimed to analyse antibiotic resistance genes in ( ) from subclinical mastitis milk in East Java Province, Indonesia. The samples consisted of subclinical mastitis milk from cows and goats. A total of 592-quarter cow...
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| Published in: | Veterinární medicína 2024-02, Vol.69 (2), p.35-41 |
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| container_title | Veterinární medicína |
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| creator | Widianingrum, Desy Cahya Silaban, Denada Grace Fanata, Wahyu Indra Duwi Khasanah, Himmatul |
| description | Antibiotics are still used to treat mastitis in dairy cows in Indonesia. This study aimed to analyse antibiotic resistance genes in
(
) from subclinical mastitis milk in East Java Province, Indonesia. The samples consisted of subclinical mastitis milk from cows and goats. A total of 592-quarter cow's milk and 71 goat's milk samples from both halves of the udder were collected from 67 farms in Lumajang, Banyuwangi, Malang, Sidoarjo, Jember, Pasuruan, Probolinggo, and Mojokerto. Subclinical mastitis samples were screened using the California mastitis test (CMT).
was identified by phenotypic and genotypic methods.
was confirmed with a primer specific to the polymerase chain reaction (PCR) technique. Gene resistance of
was tested using the multiplex-PCR (mPCR) technique with primers encoding the genes temoneira enzyme (
), oxacillinase (
), sulfhydryl variable (
), and cefotaximase-munich IV (
). These genes were chosen because mastitis treatment generally uses oxacilline and β-lactam antibiotics. All data obtained were analysed descriptively. The results show that six isolates of
(46.15%) carried a single resistance gene (
or
) and two isolates (33.33%) were confirmed as multiple drug-resistant organisms (MDROs) (
and
). The resistance genes were found in samples originating from Blitar, Banyuwangi, Lumajang, and Pasuruan Regencies. This research implies that antibiotic-resistance genes found in
on certain farms are dangerous and may allow gene transmission to other bacteria that make treatment for mastitis or other bacterial infections ineffective. |
| doi_str_mv | 10.17221/80/2023-VETMED |
| format | article |
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(
) from subclinical mastitis milk in East Java Province, Indonesia. The samples consisted of subclinical mastitis milk from cows and goats. A total of 592-quarter cow's milk and 71 goat's milk samples from both halves of the udder were collected from 67 farms in Lumajang, Banyuwangi, Malang, Sidoarjo, Jember, Pasuruan, Probolinggo, and Mojokerto. Subclinical mastitis samples were screened using the California mastitis test (CMT).
was identified by phenotypic and genotypic methods.
was confirmed with a primer specific to the polymerase chain reaction (PCR) technique. Gene resistance of
was tested using the multiplex-PCR (mPCR) technique with primers encoding the genes temoneira enzyme (
), oxacillinase (
), sulfhydryl variable (
), and cefotaximase-munich IV (
). These genes were chosen because mastitis treatment generally uses oxacilline and β-lactam antibiotics. All data obtained were analysed descriptively. The results show that six isolates of
(46.15%) carried a single resistance gene (
or
) and two isolates (33.33%) were confirmed as multiple drug-resistant organisms (MDROs) (
and
). The resistance genes were found in samples originating from Blitar, Banyuwangi, Lumajang, and Pasuruan Regencies. This research implies that antibiotic-resistance genes found in
on certain farms are dangerous and may allow gene transmission to other bacteria that make treatment for mastitis or other bacterial infections ineffective.</description><identifier>ISSN: 0375-8427</identifier><identifier>EISSN: 1805-9392</identifier><identifier>DOI: 10.17221/80/2023-VETMED</identifier><identifier>PMID: 38550619</identifier><language>eng</language><publisher>Czech Republic: Czech Academy of Agricultural Sciences (CAAS)</publisher><subject>Amides ; Antibiotic resistance ; Antibiotics ; Bacteria ; Bacterial diseases ; California mastitis test ; Cattle ; Cow's milk ; Dairy cattle ; Dairy farming ; Drug resistance ; E coli ; Escherichia coli ; Farms ; Genes ; Goat's milk ; Goats ; Mastitis ; Milk ; Multidrug resistance ; multiple drug resistant ; Original Paper ; Polymerase chain reaction ; sulfhydryl variable (shv) ; temoneira enzyme (tem) ; Udder ; zoonosis ; β-Lactam antibiotics</subject><ispartof>Veterinární medicína, 2024-02, Vol.69 (2), p.35-41</ispartof><rights>Copyright: © 2024 Widianingrum et al.</rights><rights>2024. This work is published under https://www.agriculturejournals.cz/web/about/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Copyright: © 2024 Widianingrum et al. 2024</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c442t-1592af5d91303fa2b5ded2d99b5ce7cf2ed76bc57f423978295722edd54c8b753</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10966428/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/3152978478?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,25731,27901,27902,36989,36990,44566,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38550619$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Widianingrum, Desy Cahya</creatorcontrib><creatorcontrib>Silaban, Denada Grace</creatorcontrib><creatorcontrib>Fanata, Wahyu Indra Duwi</creatorcontrib><creatorcontrib>Khasanah, Himmatul</creatorcontrib><title>Identification of antibiotic resistance genes in Escherichia coli from subclinical mastitis milk in dairy cows and goats, East Java Province</title><title>Veterinární medicína</title><addtitle>Vet Med (Praha)</addtitle><description>Antibiotics are still used to treat mastitis in dairy cows in Indonesia. This study aimed to analyse antibiotic resistance genes in
(
) from subclinical mastitis milk in East Java Province, Indonesia. The samples consisted of subclinical mastitis milk from cows and goats. A total of 592-quarter cow's milk and 71 goat's milk samples from both halves of the udder were collected from 67 farms in Lumajang, Banyuwangi, Malang, Sidoarjo, Jember, Pasuruan, Probolinggo, and Mojokerto. Subclinical mastitis samples were screened using the California mastitis test (CMT).
was identified by phenotypic and genotypic methods.
was confirmed with a primer specific to the polymerase chain reaction (PCR) technique. Gene resistance of
was tested using the multiplex-PCR (mPCR) technique with primers encoding the genes temoneira enzyme (
), oxacillinase (
), sulfhydryl variable (
), and cefotaximase-munich IV (
). These genes were chosen because mastitis treatment generally uses oxacilline and β-lactam antibiotics. All data obtained were analysed descriptively. The results show that six isolates of
(46.15%) carried a single resistance gene (
or
) and two isolates (33.33%) were confirmed as multiple drug-resistant organisms (MDROs) (
and
). The resistance genes were found in samples originating from Blitar, Banyuwangi, Lumajang, and Pasuruan Regencies. This research implies that antibiotic-resistance genes found in
on certain farms are dangerous and may allow gene transmission to other bacteria that make treatment for mastitis or other bacterial infections ineffective.</description><subject>Amides</subject><subject>Antibiotic resistance</subject><subject>Antibiotics</subject><subject>Bacteria</subject><subject>Bacterial diseases</subject><subject>California mastitis test</subject><subject>Cattle</subject><subject>Cow's milk</subject><subject>Dairy cattle</subject><subject>Dairy farming</subject><subject>Drug resistance</subject><subject>E coli</subject><subject>Escherichia coli</subject><subject>Farms</subject><subject>Genes</subject><subject>Goat's milk</subject><subject>Goats</subject><subject>Mastitis</subject><subject>Milk</subject><subject>Multidrug resistance</subject><subject>multiple drug resistant</subject><subject>Original Paper</subject><subject>Polymerase chain reaction</subject><subject>sulfhydryl variable (shv)</subject><subject>temoneira enzyme (tem)</subject><subject>Udder</subject><subject>zoonosis</subject><subject>β-Lactam antibiotics</subject><issn>0375-8427</issn><issn>1805-9392</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNpdks1u1DAUhSNERYfCmh2yxIYFYZxrO4lXCJUAUxXBorC1HP_MeEjs1s4M6lvwCH2WPhmemVJRVpauz_187_EpihcVfls1ANW8xXPAQMof3cWX7sOjYla1mJWccHhczDBpWNlSaI6LpymtMQZeYf6kOCYtY7iu-Kz4vdDGT846JScXPAr29kbmQu_C5BSKJrk0Sa8MWhpvEnL-9gZ1Sa1MdGrlJFJhcMjGMKK06dXgfCYNaJRpcpNLaHTDz32Tli5eZ_WvhKTXaBnklN6gLuvQmdxK9C2GrcvvPCuOrBySeX53nhTfP3YXp5_L86-fFqfvz0tFKUxlxThIyzSvCCZWQs-00aA575kyjbJgdFP3ijWWAuFNC5xlv4zWjKq2bxg5KRYHrg5yLS6jG2W8FkE6sS-EuBQyZgsGI6jmRNUajGU9tX3NM4ADUMp73UgFmfXuwLrc9KPRKjsa5fAA-vDGu5VYhq3Iv1HXFNpMeH1HiOFqY9IkRpeUGQbpTdgkQTBAXqBud9JX_0nXYRN99kqQikHelTY71fygUjGkFI29n6bCYh8d0WKxi444RCd3vPx3iXv936yQP_1hxJU</recordid><startdate>202402</startdate><enddate>202402</enddate><creator>Widianingrum, Desy Cahya</creator><creator>Silaban, Denada Grace</creator><creator>Fanata, Wahyu Indra Duwi</creator><creator>Khasanah, Himmatul</creator><general>Czech Academy of Agricultural Sciences (CAAS)</general><general>Czech Academy of Agricultural Sciences</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>HCIFZ</scope><scope>M0K</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>202402</creationdate><title>Identification of antibiotic resistance genes in Escherichia coli from subclinical mastitis milk in dairy cows and goats, East Java Province</title><author>Widianingrum, Desy Cahya ; Silaban, Denada Grace ; Fanata, Wahyu Indra Duwi ; Khasanah, Himmatul</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c442t-1592af5d91303fa2b5ded2d99b5ce7cf2ed76bc57f423978295722edd54c8b753</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Amides</topic><topic>Antibiotic resistance</topic><topic>Antibiotics</topic><topic>Bacteria</topic><topic>Bacterial diseases</topic><topic>California mastitis test</topic><topic>Cattle</topic><topic>Cow's milk</topic><topic>Dairy cattle</topic><topic>Dairy farming</topic><topic>Drug resistance</topic><topic>E coli</topic><topic>Escherichia coli</topic><topic>Farms</topic><topic>Genes</topic><topic>Goat's milk</topic><topic>Goats</topic><topic>Mastitis</topic><topic>Milk</topic><topic>Multidrug resistance</topic><topic>multiple drug resistant</topic><topic>Original Paper</topic><topic>Polymerase chain reaction</topic><topic>sulfhydryl variable (shv)</topic><topic>temoneira enzyme (tem)</topic><topic>Udder</topic><topic>zoonosis</topic><topic>β-Lactam antibiotics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Widianingrum, Desy Cahya</creatorcontrib><creatorcontrib>Silaban, Denada Grace</creatorcontrib><creatorcontrib>Fanata, Wahyu Indra Duwi</creatorcontrib><creatorcontrib>Khasanah, Himmatul</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Agricultural Science Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>SciTech Premium Collection</collection><collection>Agriculture Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Veterinární medicína</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Widianingrum, Desy Cahya</au><au>Silaban, Denada Grace</au><au>Fanata, Wahyu Indra Duwi</au><au>Khasanah, Himmatul</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of antibiotic resistance genes in Escherichia coli from subclinical mastitis milk in dairy cows and goats, East Java Province</atitle><jtitle>Veterinární medicína</jtitle><addtitle>Vet Med (Praha)</addtitle><date>2024-02</date><risdate>2024</risdate><volume>69</volume><issue>2</issue><spage>35</spage><epage>41</epage><pages>35-41</pages><issn>0375-8427</issn><eissn>1805-9392</eissn><abstract>Antibiotics are still used to treat mastitis in dairy cows in Indonesia. This study aimed to analyse antibiotic resistance genes in
(
) from subclinical mastitis milk in East Java Province, Indonesia. The samples consisted of subclinical mastitis milk from cows and goats. A total of 592-quarter cow's milk and 71 goat's milk samples from both halves of the udder were collected from 67 farms in Lumajang, Banyuwangi, Malang, Sidoarjo, Jember, Pasuruan, Probolinggo, and Mojokerto. Subclinical mastitis samples were screened using the California mastitis test (CMT).
was identified by phenotypic and genotypic methods.
was confirmed with a primer specific to the polymerase chain reaction (PCR) technique. Gene resistance of
was tested using the multiplex-PCR (mPCR) technique with primers encoding the genes temoneira enzyme (
), oxacillinase (
), sulfhydryl variable (
), and cefotaximase-munich IV (
). These genes were chosen because mastitis treatment generally uses oxacilline and β-lactam antibiotics. All data obtained were analysed descriptively. The results show that six isolates of
(46.15%) carried a single resistance gene (
or
) and two isolates (33.33%) were confirmed as multiple drug-resistant organisms (MDROs) (
and
). The resistance genes were found in samples originating from Blitar, Banyuwangi, Lumajang, and Pasuruan Regencies. This research implies that antibiotic-resistance genes found in
on certain farms are dangerous and may allow gene transmission to other bacteria that make treatment for mastitis or other bacterial infections ineffective.</abstract><cop>Czech Republic</cop><pub>Czech Academy of Agricultural Sciences (CAAS)</pub><pmid>38550619</pmid><doi>10.17221/80/2023-VETMED</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Veterinární medicína, 2024-02, Vol.69 (2), p.35-41 |
| issn | 0375-8427 1805-9392 |
| language | eng |
| recordid | cdi_doaj_primary_oai_doaj_org_article_4d93c6d2ef5b4fb698b7922449bd7ac2 |
| source | Publicly Available Content Database; PubMed Central |
| subjects | Amides Antibiotic resistance Antibiotics Bacteria Bacterial diseases California mastitis test Cattle Cow's milk Dairy cattle Dairy farming Drug resistance E coli Escherichia coli Farms Genes Goat's milk Goats Mastitis Milk Multidrug resistance multiple drug resistant Original Paper Polymerase chain reaction sulfhydryl variable (shv) temoneira enzyme (tem) Udder zoonosis β-Lactam antibiotics |
| title | Identification of antibiotic resistance genes in Escherichia coli from subclinical mastitis milk in dairy cows and goats, East Java Province |
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