Regulation of V-ATPase by Jasmonic Acid: Possible Role of Persulfidation

Vacuolar H+-translocating ATPase (V-ATPase) is a proton pump crucial for plant growth and survival. For this reason, its activity is tightly regulated, and various factors, such as signaling molecules and phytohormones, may be involved in this process. The aim of this study was to explain the role o...

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Bibliographic Details
Published in:International journal of molecular sciences 2023-09, Vol.24 (18), p.13896
Main Authors: Zboińska, Magdalena, Romero, Luis C., Gotor, Cecilia, Kabała, Katarzyna
Format: Article
Language:English
Subjects:
Acids
Adenosine triphosphatase
Analysis
cadmium
Enzymes
Ethylenediaminetetraacetic acid
Gene expression
H2O2
H2S
jasmonic acid
Kinases
Membrane proteins
persulfidation
Polyamines
Proteins
proton pump
Protons
Pumping machinery industry
Signal transduction
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Summary:Vacuolar H+-translocating ATPase (V-ATPase) is a proton pump crucial for plant growth and survival. For this reason, its activity is tightly regulated, and various factors, such as signaling molecules and phytohormones, may be involved in this process. The aim of this study was to explain the role of jasmonic acid (JA) in the signaling pathways responsible for the regulation of V-ATPase in cucumber roots and its relationship with other regulators of this pump, i.e., H2S and H2O2. We analyzed several aspects of the JA action on the enzyme, including transcriptional regulation, modulation of protein levels, and persulfidation of selected V-ATPase subunits as an oxidative posttranslational modification induced by H2S. Our results indicated that JA functions as a repressor of V-ATPase, and its action is related to a decrease in the protein amount of the A and B subunits, the induction of oxidative stress, and the downregulation of the E subunit persulfidation. We suggest that both H2S and H2O2 may be downstream components of JA-dependent negative proton pump regulation. The comparison of signaling pathways induced by two negative regulators of the pump, JA and cadmium, revealed that multiple pathways are involved in the V-ATPase downregulation in cucumber roots.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms241813896