Concanavalin A photocross-linked affinity cryogels for the purification of horseradish peroxidase

The present study describes an easy and efficient procedure for the purification of horseradish peroxidase from horseradish roots. For this purpose, supermacroporous cryogels having Concanavalin A were prepared by photosensitive cross-linking polymerization. Horseradish peroxidase binding and elutio...

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Bibliographic Details
Published in:Adsorption science & technology 2018-05, Vol.36 (3-4), p.1199-1212
Main Authors: Keçili, Rüstem, Çelikoğlu, Umut, Mil, Sevgi, Ersöz, Arzu, Say, Rıdvan
Format: Article
Language:English
Subjects:
Binding
Concanavalin A
Crosslinking
Dichroism
Elution
Peroxidase
Photosensitivity
Purification
Roots
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Summary:The present study describes an easy and efficient procedure for the purification of horseradish peroxidase from horseradish roots. For this purpose, supermacroporous cryogels having Concanavalin A were prepared by photosensitive cross-linking polymerization. Horseradish peroxidase binding and elution from the prepared cryogels were carried out changing various parameters such as initial peroxidase concentration and pH. The best binding performance was obtained at pH 7.0. The maximum horseradish peroxidase binding of the cryogels was found to be 3.85 mg g−1 cryogel. Horseradish peroxidase purification from crude extract resulted in 115.1-fold. SDS-PAGE analysis and circular dichroism measurements indicated that the horseradish peroxidase purification from horseradish roots was successfully carried out.
ISSN:0263-6174
2048-4038
DOI:10.1177/0263617418760637