Optical Tweezers with Integrated Multiplane Microscopy (OpTIMuM): a new tool for 3D microrheology

We introduce a novel 3D microrheology system that combines for the first time Optical Tweezers with Integrated Multiplane Microscopy (OpTIMuM). The system allows the 3D tracking of an optically trapped bead, with ~ 20 nm accuracy along the optical axis. This is achieved without the need for a high p...

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Bibliographic Details
Published in:Scientific reports 2021-03, Vol.11 (1), p.5614-5614, Article 5614
Main Authors: Matheson, Andrew B., Paterson, Lynn, Wright, Amanda J., Mendonca, Tania, Tassieri, Manlio, Dalgarno, Paul A.
Format: Article
Language:English
Subjects:
631/1647
631/57
639/301
639/624
639/766
Calibration
Humanities and Social Sciences
Microscopy
multidisciplinary
Optical properties
Science
Science (multidisciplinary)
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Summary:We introduce a novel 3D microrheology system that combines for the first time Optical Tweezers with Integrated Multiplane Microscopy (OpTIMuM). The system allows the 3D tracking of an optically trapped bead, with ~ 20 nm accuracy along the optical axis. This is achieved without the need for a high precision z-stage, separate calibration sample, nor a priori knowledge of either the bead size or the optical properties of the suspending medium. Instead, we have developed a simple yet effective in situ spatial calibration method using image sharpness and exploiting the fact we image at multiple planes simultaneously. These features make OpTIMuM an ideal system for microrheology measurements, and we corroborate the effectiveness of this novel microrheology tool by measuring the viscosity of water in three dimensions, simultaneously.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-021-85013-y