Expression of p-PPARγ in the aging thoracic aorta of spontaneously hypertensive rat and inhibitory effect of rosiglitazone

To investigate the expression of phosphorylated peroxisome proliferators-activated receptor γ (p-PPARγ) in the aging thoracic aorta of spontaneously hypertensive rat (SHR) and the inhibitory effect of rosiglitazone on the phosphorylation of PPARγ. 16, 32 and 64 week-old Wistar-Kyoto rats (WKY) and S...

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Bibliographic Details
Published in:Asian Pacific journal of tropical biomedicine 2014-12, Vol.4 (12), p.977-981
Main Authors: Yuan, Hai-Feng, Niu, Xiao-Lin, Gao, Deng-Feng, Hao, Guang-Hua, Song, An-Qi, Wei, Jin
Format: Article
Language:English
Subjects:
Aging
P-PPARγ
Rosiglitazone
Spontaneously hypertensive rat
Thoracic aorta
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Summary:To investigate the expression of phosphorylated peroxisome proliferators-activated receptor γ (p-PPARγ) in the aging thoracic aorta of spontaneously hypertensive rat (SHR) and the inhibitory effect of rosiglitazone on the phosphorylation of PPARγ. 16, 32 and 64 week-old Wistar-Kyoto rats (WKY) and SHR were randomly and respectively divided into WKY, SHR and SHR+rosiglitazone group (9 in each group). The rats in SHR+rosiglitazone group were treated with rosiglitazone (5 mg/kg, intragastrically) for 56 d, whereas normal saline was applied in WKY and SHR groups. Systolic blood pressure (SBP) of rats was measured by tail cuff method. Histopathological damage of thoracic aorta was analyzed using Hematoxylin-Eosin (HE) staining. Immunohistochemical staining and western blot were performed to test the level of p-PPARγ protein in the thoracic aorta arising from each group. The SBP in 16, 32 and 64 week-old SHR were significantly higher as compared with those in matched WKY rats (P
ISSN:2221-1691
2588-9222
DOI:10.12980/APJTB.4.201414B416