The Repression of Matrix Metalloproteinases and Cytokine Secretion in Glioblastoma by Targeting K+ Channel

• Published in: Basic and clinical neuroscience 2021-11, Vol.12 (6), p.737-744
• Main Authors: Saadat, Farshid, Zareighane, Zohreh, Safavifar, Farnaz, Jalali, Seyedeh Zohreh, Berahmeh, Azar, Khorramizadeh, Mohammad Reza
• Format: Article
• Language: English
• Subjects: 4-aminopyridine; Astrocytes; Brain tumors; Cell viability; Channel gating; Cytokines; Cytotoxicity; Enzyme-linked immunosorbent assay; Gelatin; Gelatinase B; Glioblastoma; Immunopathogenesis; Interleukin 1; Interleukin 6; Matrix metalloproteinase; matrix metalloproteinases; Metastases; Potassium; potassium channels; Potassium channels (voltage-gated); Research Paper; voltage-gated
• ISSN: 2228-7442; 2008-126X; 2228-7442
• DOI: 10.32598/bcn.2021.1693.1

Introduction: Glioblastoma is an aggressive human brain malignancy with poorly understood pathogenesis. Voltage-gated potassium (Kv) channels and Matrix Metalloproteinases (MMPs) are highly expressed in malignant tumors and involved in the progression and metastasis of glioblastoma. This study aimed to determine whether a voltage-dependent potassium channel blocker could modulate astrocytes as a cell involved in the immunopathogenesis of glioblastoma. Methods: The cytotoxic effect of 4-Aminopyridine (4-AP) at different doses in the cell model of glioblastoma was measured by MTT assay. The ELISA technique and gelatin zymography were used to assess cytokine levels and MMP-9 after 4-AP treatment. Results: Cytotoxicity analysis data indicated that cell viability reduced by increasing 4-AP level and cell growth decreased gradually by removing 4-AP from the cell medium. 4-AP inhibits the secretion of IL-6 and IL-1 (P