Graphene Oxide Tablets for Sample Preparation of Drugs in Biological Fluids: Determination of Omeprazole in Human Saliva for Liquid Chromatography Tandem Mass Spectrometry

In this study, a novel sort of sample preparation sorbent was developed, by preparing thin layer graphene oxide tablets (GO-Tabs) utilizing a mixture of graphene oxide and polyethylene glycol on a polyethylene substrate. The GO-Tabs were used for extraction and concentration of omeprazole (OME) in h...

Full description

Saved in:
Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2019-03, Vol.24 (7), p.1191
Main Authors: Zohdi, Zeynab, Hashemi, Mahdi, Uheida, Abdusalam, Moein, Mohammad Mahdi, Abdel-Rehim, Mohamed
Format: Article
Language:English
Subjects:
Acids
Calibration
Chromatography
GO-Tabs
Graphene
graphene oxide
Ions
Liquid chromatography
Mass spectrometry
Mass spectroscopy
Medicin och hälsovetenskap
Omeprazole
Plasma
Polyethylene films
Polyethylene glycol
Positive ions
Public transportation
Quality control
Saliva
Sample preparation
Scientific imaging
Sorbents
Spectroscopy
Tablets
tandem mass spectrometry
Water purification
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In this study, a novel sort of sample preparation sorbent was developed, by preparing thin layer graphene oxide tablets (GO-Tabs) utilizing a mixture of graphene oxide and polyethylene glycol on a polyethylene substrate. The GO-Tabs were used for extraction and concentration of omeprazole (OME) in human saliva samples. The determination of OME was carried out using liquid chromatography-tandem mass spectrometry (LC⁻MS/MS) under gradient LC conditions and in the positive ion mode (ESI+) with mass transitions of m/z 346.3→198.0 for OME and / 369.98→252.0 for the internal standard. Standard calibration for the saliva samples was in the range of 2.0⁻2000 nmol L . Limits of detection and quantification were 0.05 and 2.0 nmol L , respectively. Method validation showed good method accuracy and precision; the inter-day precision values ranged from 5.7 to 8.3 (%RSD), and the accuracy of determinations varied from -11.8% to 13.3% (% deviation from nominal values). The extraction recovery was 60%, and GO-Tabs could be re-used for more than ten extractions without deterioration in recovery. In this study, the determination of OME in real human saliva samples using GO-Tab extraction was validated.
ISSN:1420-3049
1431-5157
1420-3049
DOI:10.3390/molecules24071191