Preliminary study on microR-148a and microR-10a in dermal papilla cells of Hu sheep

• Published in: BMC genetics 2019-08, Vol.20 (1), p.70-70, Article 70
• Main Authors: Lv, Xiaoyang, Gao, Wen, Jin, Chengyan, Wang, Lihong, Wang, Yue, Chen, Weihao, Zou, Shuangxia, Huang, Sainan, Li, Zhifeng, Wang, Jinyu, Sun, Wei
• Format: Article
• Language: English
• Subjects: 3' Untranslated Regions; Animals; Bioinformatics; BMP7; Bone morphogenetic protein 7; Bone morphogenetic proteins; Cell proliferation; Cells, Cultured; China; Cholecystokinin; Computational biology; Dermal papilla cell; Dermis; Dermis - metabolism; DNA microarrays; Follicles; Gene Expression Profiling; Genes; Genes, Reporter; Genetic aspects; growth and development; Hair; hair follicles; Hair loss; high-throughput nucleotide sequencing; Hu sheep; Humans; Inhibition; Journalists; Luciferase; MicroRNA; MicroRNAs - genetics; miR-10a; miR-148a; Next-generation sequencing; Physiological aspects; Polymerase chain reaction; quantitative polymerase chain reaction; Reproduction; reproductive performance; reverse transcriptase polymerase chain reaction; Sheep; Sheep - genetics; Skin; Smad2 protein; Smad3 protein; Smad4 protein; Smad5 protein; transforming growth factor beta; Transforming growth factors; Western blotting
• ISSN: 1471-2156; 1471-2156
• DOI: 10.1186/s12863-019-0770-8

Hu sheep, a unique Chinese breed with high reproductive performance, are also well known for their rare white lambskin in China. The quality of lambskin is affected by hair follicles, and dermal papilla cells are an important component of hair follicles that plays a key role in hair follicle growth and development. This study helps elucidate the effect of miR-148a and miR-10a on hair follicle growth and development. Based on the results of gene chip and high-throughput sequencing, bone morphogenetic protein 7 (BMP7) was used as a research object. Bioinformatics analysis and the dual-luciferase reporter system indicated that, along with Western blot and quantitative real-time polymerase chain reaction (qRT-PCR) that miR-148a and miR-10a target relationships with BMP7. BMP7 was the target gene both for miR-148a and miR-10a by the dual-luciferase reporter system and Western blot. Hu sheep dermal papilla cells were successfully isolated and purified, and after transfecting miR-148a/miR-10a mimics and inhibitors into dermal papilla cells, a Cell Counting Kit-8 (CCK-8) was used to determine that miR-148a/miR-10a inhibited the proliferation of Hu sheep dermal papilla cells. In addition, after the overexpression of miR-148a, the expression levels of Smad3 (P