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Oocyte Cryopreservation in Domestic Animals and Humans: Principles, Techniques and Updated Outcomes

Oocyte cryopreservation plays important roles in basic research and the application of models for genetic preservation and in clinical situations. This technology provides long-term storage of gametes for genetic banking and subsequent use with other assisted reproductive technologies. Until recentl...

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Published in:Animals (Basel) 2021-10, Vol.11 (10), p.2949
Main Authors: Tharasanit, Theerawat, Thuwanut, Paweena
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Language:English
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description Oocyte cryopreservation plays important roles in basic research and the application of models for genetic preservation and in clinical situations. This technology provides long-term storage of gametes for genetic banking and subsequent use with other assisted reproductive technologies. Until recently, oocytes have remained the most difficult cell type to freeze, as the oocytes per se are large with limited surface area to cytoplasm ratio. They are also highly sensitive to damage during cryopreservation, and therefore the success rate of oocyte cryopreservation is generally poor when compared to noncryopreserved oocytes. Although advancement in oocyte cryopreservation has progressed rapidly for decades, the improvement of cryosurvival and clinical outcomes is still required. This review focuses on the principles, techniques, outcomes and prospects of oocyte cryopreservation in domestic animals and humans.
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subjects animal
Animals
Apoptosis
Cell viability
Cooling
Cryoinjury
Cryopreservation
Cytoplasm
Domestic animals
Embryos
Fertility
Freezing
Gametes
Gametocytes
human
In vitro fertilization
Infertility
Laboratories
Molecular weight
oocyte
Oocytes
Ovaries
Permeability
Physiology
Pregnancy
Principles
Reproductive technologies
Reproductive technology
Review
Success
Technology
title Oocyte Cryopreservation in Domestic Animals and Humans: Principles, Techniques and Updated Outcomes
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