On-Site Pilot-Scale Microalgae Cultivation Using Industrial Wastewater for Bioenergy Production: A Case Study towards Circular Bioeconomy

• Published in: Bioengineering (Basel) 2023-11, Vol.10 (12), p.1339
• Main Authors: Abraham, Juliana, Abimbola, Tobi, Braida, Washington J, Terracciano, Amalia, Su, Tsan-Liang, Christodoulatos, Christos, Koutsospyros, Agamemnon, RoyChowdhury, Abhishek, Smolinski, Benjamin, Lawal, Adeniyi
• Format: Article
• Language: English
• Subjects: Algae; Aquatic microorganisms; areal biomass productivity; Biodiesel fuels; Bioengineering; Biomass; Biomass energy; carbon capture; Case studies; Cyanobacteria; Dissolved oxygen; energy potential; Industrial wastes; industrial wastewater valorization; Life cycle analysis; Life cycle assessment; Life cycles; Light intensity; Luminous intensity; Nitrates; Nutrients; Oils & fats; On-line systems; on-site pilot scale; Onsite; Physiological aspects; Ponds; Production processes; Renewable energy; Sensors; sustainability; Wastewater
• ISSN: 2306-5354; 2306-5354
• DOI: 10.3390/bioengineering10121339

This case study assesses the valorization of industrial wastewater streams for bioenergy generation in an industrial munition facility. On-site pilot-scale demonstrations were performed to investigate the feasibility of algal growth in the target wastewater on a larger outdoor scale. An exploratory field study followed by an optimized one were carried out using two 1000 L open raceway ponds deployed within a greenhouse at an industrial munition facility. An online system allowed for constant monitoring of operational parameters such as temperature, pH, light intensity, and dissolved oxygen within the ponds. The original algal seed evolved into an open-air resilient consortium of green microalgae and cyanobacteria that were identified and characterized successfully. Weekly measurements of the level of nutrients in pond liquors were performed along with the determination of the algal biomass to quantitatively evaluate growth yields. After harvesting algae from the ponds, the biomass was concentrated and evaluated for oil content and biochemical methane potential (BMP) to provide an estimate of the algae-based energy production. Additionally, the correlation among biomass, culturing conditions, oil content, and BMP was evaluated. The higher average areal biomass productivity achieved during the summer months was 23.9 ± 0.9 g/m d, with a BMP of 350 scc/gVS. An oil content of 22 wt.% was observed during operation under low nitrogen loads. Furthermore, a technoeconomic analysis and life cycle assessment demonstrated the viability of the proposed wastewater valorization scenario and aided in optimizing process performance towards further scale-up.