Effects of the preservation medium and storage duration of domestic cat ovaries on the maturational and developmental competence of oocytes in vitro

• Published in: Journal of Reproduction and Development 2022, Vol.68(2), pp.160-164
• Main Authors: YOSHIDA, Takumi, ALAM, Md Emtiaj, HANAFUSA, Keisuke, TSUJIMOTO, Yasunori, TSUKAMOTO, Masaya, KANEGI, Ryoji, INABA, Toshio, SUGIURA, Kikuya, HATOYA, Shingo
• Format: Article
• Language: English
• Subjects: Blastocyst; Blastocysts; Cold storage; Cryopreservation; Feline oocyte; In vitro culture; In vitro maturation; Oocytes; Ovaries; Preservation; Technology Report
• ISSN: 0916-8818; 1348-4400
• DOI: 10.1262/jrd.2021-084

We examined the effectiveness of saline, Euro-Collins solution (EC), and ET-Kyoto solution (ET-K) as preservation media for the cold storage of feline ovaries. Ovaries were maintained in these media at 4°C for 24, 48, or 72 h until oocyte retrieval. The ET-K group exhibited a higher oocyte maturation rate than the saline group after 72 h of storage. Moreover, ET-K could sustain the competence of the feline oocytes to cleave after 48 h, and the morula formation rate of the ET-K group was higher than that of the other groups after 24 and 48 h. Furthermore, the ET-K group exhibited a higher blastocyst formation rate than the other groups after storage for 24 h, and only ET-K retained the developmental competence in blastocysts after 48 h of storage. In addition, regarding the cell numbers of the blastocysts, there was no significant difference among the tested groups. In conclusion, our results indicate that ET-K is a suitable preservation medium for feline ovaries.