Pollen Count Dynamics in Rapeseed Stamens in Early Spring

Pollen contains vegetative and generative cells that influence the seed set. Accurate pollen assessments help understand the breeding biology of oilseed rape. In order to evaluate the number of pollen grains in a large number of samples, an effective method should be designed. A new method was estab...

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Bibliographic Details
Published in:Journal of Apicultural Science 2023-12, Vol.67 (2), p.103-114
Main Authors: Wei, Zhijun, Liu, Ran, Ding, Guiling, Jiang, Yusuo, Huang, Jiaxing
Format: Article
Language:English
Subjects:
Absorbance
Anthers
brassica napus
Cultivars
Flowering
oilseed rape
Oilseeds
Plant breeding
Pollen
pollen counting
pollen deposition
Rape plants
Rapeseed
Seed set
Spectroscopy
Stamens
Sucrose
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Summary:Pollen contains vegetative and generative cells that influence the seed set. Accurate pollen assessments help understand the breeding biology of oilseed rape. In order to evaluate the number of pollen grains in a large number of samples, an effective method should be designed. A new method was established to evaluate the pollen counts of five oilseed rape cultivars. This method mainly relies on 5% sucrose + 0.1% agar solution to suspend the pollen and uses a standard curve corresponding to the absorbance and the number of pollen grains to estimate the number of pollen grains. The linear fit formula of this standard curve was precision, and the R value between the pollen count and absorbance reached 0.998. Furthermore, the variation in the pollen counts at three flowering stages and on two kinds of stamens was assessed. The pollen count per flower varied significantly among oilseed rape cultivars and flowering stages (GLM: p
ISSN:2299-4831
1643-4439
2299-4831
DOI:10.2478/jas-2023-0008