Isolation and characterization of a halophilic Modicisalibacter sp. strain Wilcox from produced water

• Published in: Scientific reports 2021-03, Vol.11 (1), p.6943-14, Article 6943
• Main Authors: Marsh, William S., Heise, Brenden W., Krzmarzick, Mark J., Murdoch, Robert W., Fathepure, Babu Z.
• Format: Article
• Language: English
• Subjects: 631/326; 631/326/171; 704/172; Aliphatic compounds; Aliphatic hydrocarbons; Aromatic hydrocarbons; Benzene; Benzoic acid; Biodegradation; Biodegradation, Environmental; Biphenyl; Carbon; Carbon sources; Detoxification; Ethylbenzene; Genome, Bacterial; Genomes; Halomonadaceae - genetics; Halomonadaceae - isolation & purification; Halomonadaceae - metabolism; Heavy metals; Hexadecane; Humanities and Social Sciences; Hydrocarbon-degrading bacteria; Hydrocarbons; Hydrocarbons - metabolism; Industrial Waste; Laboratory culture; multidisciplinary; Oil and gas fields; Oil and Gas Fields - microbiology; Petroleum Pollution; Phenols; Phylogeny; Protein transport; rRNA 16S; Science; Science (multidisciplinary); Sodium chloride; Toluene
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-021-86196-0

We report the isolation a halophilic bacterium that degrades both aromatic and aliphatic hydrocarbons as the sole sources of carbon at high salinity from produced water. Phylogenetic analysis of 16S rRNA-gene sequences shows the isolate is a close relative of Modicisalibacter tunisiensis isolated from an oil-field water in Tunisia. We designate our isolate as Modicisalibacter sp. strain Wilcox. Genome analysis of strain Wilcox revealed the presence of a repertoire of genes involved in the metabolism of aliphatic and aromatic hydrocarbons. Laboratory culture studies corroborated the predicted hydrocarbon degradation potential. The strain degraded benzene, toluene, ethylbenzene, and xylenes at salinities ranging from 0.016 to 4.0 M NaCl, with optimal degradation at 1 M NaCl. Also, the strain degraded phenol, benzoate, biphenyl and phenylacetate as the sole sources of carbon at 2.5 M NaCl. Among aliphatic compounds, the strain degraded n-decane and n-hexadecane as the sole sources of carbon at 2.5 M NaCl. Genome analysis also predicted the presence of many heavy metal resistance genes including genes for metal efflux pumps, transport proteins, and enzymatic detoxification. Overall, due to its ability to degrade many hydrocarbons and withstand high salt and heavy metals, strain Wilcox may prove useful for remediation of produced waters.