Does mechanical damage on soybean induces the production of flavonoids?

• Published in: Anais da Academia Brasileira de Ciências 2018-10, Vol.90 (4), p.3415-3422
• Main Authors: Silva, Paulo Luiz DA, Cordeiro, Gláucia, Silva, Carolina R DA, Barros, Rafael A, Silva, Camila R DA, Zanuncio, José C, Campos, Wellington G, Oliveira, Maria G A
• Format: Article
• Language: English
• Subjects: Chromatography, High Pressure Liquid; flavonoid; Glycine max; Glycine max - chemistry; herbivory; Isoflavones - analysis; mechanical damage; Mechanical Phenomena; MULTIDISCIPLINARY SCIENCES; Plant Leaves - chemistry; Plant Physiological Phenomena; Quercetin - analysis; Rutin - analysis; secondary metabolite
• ISSN: 0001-3765; 1678-2690; 1678-2690
• DOI: 10.1590/0001-3765201820170850

The response of plants to grazing includes the production of chemical defense compounds such as proteases inhibitors and secondary metabolites as flavonoids, which makes them less palatable to feeding and negatively affecting the physiology of insects. The aim of this study was to evaluate the phytochemical response of soybean cultivars (Glycine max (L.) Merrill) resistant (IAC-17, IAC-24) and susceptible (IAC-P1) to insects after mechanical damage. These cultivars were mechanically injured, and after 24 hours samples of these plants were analyzed by HPLC to identify and quantify flavonoids. The flavonoids daidzein, quercetin, and rutin were quantified, with the highest concentration of daidzin in soybean cultivars after mechanical damage. Rutin was biosynthesized by IAC-24. The cultivars IAC-PL1, IAC-17, and IAC-24 did not show a flavonoid response to mechanical damage. The soybean cultivars are not dependent on mechanical damage to produce flavonoids.