Discovery and Characterization of Chemical Compounds That Inhibit the Function of Bacterial Neuraminidase from Codonopsis ussuriensis

Codonopsis ussuriensis (Rupr. and Maxim.) Hemsl is a medicinal herb commonly found in Korea, Japan, and the Russian Far East. However, its chemical composition and bioactivity have not been extensively studied. Thus, in this study, we aimed to examine and characterize the major components of the pla...

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Bibliographic Details
Published in:Applied sciences 2022-06, Vol.12 (12), p.6254
Main Authors: Koo, Sung Cheol, Woo, Hyun Sim, Kim, Jae Woo, Kim, Yeong-Su, Kim, Jeong Yoon, Kim, Jang Hoon, Yu, Jin, Kim, Young Chang, Kim, Dae Wook
Format: Article
Language:English
Subjects:
Acids
Bacteria
bacterial neuraminidase
Biological activity
Chemical composition
Chemical compounds
Chromatography
Codonopsis
Codonopsis ussuriensis
Enzyme kinetics
Exo-a-sialidase
Gas flow
Herbal medicine
Liquid chromatography
Mass spectrometry
Mass spectroscopy
mixed type-I inhibition
NMR
Nuclear magnetic resonance
Software
tangshenoside I
ussurienoside I
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Summary:Codonopsis ussuriensis (Rupr. and Maxim.) Hemsl is a medicinal herb commonly found in Korea, Japan, and the Russian Far East. However, its chemical composition and bioactivity have not been extensively studied. Thus, in this study, we aimed to examine and characterize the major components of the plant’s roots. Liquid chromatography with high-resolution mass spectrometry (LC-HRMS) was used to identify the components of the crude extracts. The compounds that were identified were named ussurienoside I (1) and tangshenoside I (2). The identified compounds were tested in vitro for inhibitory action against bacterial neuraminidase, and the mechanisms of inhibition were revealed. Compound 1 significantly inhibited bacterial neuraminidase activity in a dose-dependent manner (IC50 = 56.0 μM). The neuraminidase inhibitor (compound 1) exhibited mixed type-I kinetic characteristics. The LC-HRMS data showed that the root extracts contained eight compounds. The findings of this study may aid in the development of bacterial neuraminidase inhibitors with medicinal potential.
ISSN:2076-3417
2076-3417
DOI:10.3390/app12126254