Starch Digestion Enhances Bioaccessibility of Anti-Inflammatory Polyphenols from Borlotti Beans ( Phaseolus vulgaris )

• Published in: Nutrients 2020-01, Vol.12 (2), p.295
• Main Authors: Perez-Hernandez, Lucia Margarita, Nugraheni, Kartika, Benohoud, Meryem, Sun, Wen, Hernández-Álvarez, Alan Javier, Morgan, Michael R A, Boesch, Christine, Orfila, Caroline
• Format: Article
• Language: English
• Subjects: Acids; Anti-inflammatory agents; Anti-Inflammatory Agents - pharmacokinetics; anti-inflammatory effect; Beans; bioaccessibility; Bioavailability; Biological Availability; Cooking; Digestion; domestic processing; Endosperm; Enzymes; Flavones; Flavonoids; Fluorescence; Fluorescence microscopy; Food Handling; Gallic acid; Humans; Hydroxycinnamic acid; il1β; in vitro digestion; Inflammation; inos; Interleukin 1; Macrophages; Macrophages - drug effects; mRNA; Nitric-oxide synthase; Phaseolus - chemistry; Phaseolus vulgaris; Phytochemicals; Polyphenols; Polyphenols - pharmacokinetics; Proteins; Seed coats; Starch; Starch - metabolism; starch digestion
• ISSN: 2072-6643; 2072-6643
• DOI: 10.3390/nu12020295

The consumption of beans has been associated with chronic disease prevention which may be attributed to the polyphenols present in the seed coat and endosperm. However, their bioaccessibility is likely to be limited by interactions with bean matrix components, including starch, protein and fibre. The aim of this project was to evaluate the effect of domestic processing and enzymatic digestion on the bioaccessibility of polyphenols from Borlotti beans ( ) and to test their anti-inflammatory properties in a macrophage cell model. digestion of cooked beans released twenty times more polyphenols (40.4 ± 2.5 mg gallic acid equivalents (GAE)/g) than domestic processing (2.22 ± 0.1 mg GAE/g), with starch digestion contributing to the highest release (30.9 ± 0.75 mg GAE/g). Fluorescence microscopy visualization of isolated bean starch suggests that polyphenols are embedded within the granule structure. LC-MS analysis showed that cooked Borlotti bean contain flavonoids, flavones and hydroxycinnamic acids, and cooked bean extracts exerted moderate anti-inflammatory effects by decreasing mRNA levels of IL1β and iNOS by 25% and 40%, respectively. In conclusion, the bioaccessibility of bean polyphenols is strongly enhanced by starch digestion. These polyphenols may contribute to the health benefits associated with bean consumption.