Structural and Dynamic Analysis of Leaf-Associated Fungal Community of Walnut Leaves Infected by Leaf Spot Disease Based Illumina High-Throughput Sequencing Technology

Leaf-associated microbiota is vital in plant-environment interactions and is the basis for micro-ecological regulation. However, there are no studies on the direct differences in microbial community composition between disease-susceptible and healthy walnut leaves. This study collected five samples...

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Bibliographic Details
Published in:Polish journal of microbiology 2022-09, Vol.71 (3), p.429-441
Main Authors: Wang, Shiwei, Tan, Yu, Li, Shujiang, Zhu, Tianhui
Format: Article
Language:English
Subjects:
Community composition
Community structure
Disease control
Diversity indices
dynamic analysis
Fungi
high throughput sequencing
leaf-associated fungi
Leafspot
Leaves
Microbial activity
Microbiota
Microorganisms
Next-generation sequencing
Original Paper
Plant diseases
Population dynamics
spot disease
walnut
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Summary:Leaf-associated microbiota is vital in plant-environment interactions and is the basis for micro-ecological regulation. However, there are no studies on the direct differences in microbial community composition between disease-susceptible and healthy walnut leaves. This study collected five samples of healthy and infected leaves (all leaves with abnormal spots were considered diseased leaves) from May to October 2018. Differences in fungal diversity (Chao1 index, Shannon index, and Simpson index) and community structure were observed by sequencing and analyzing diseased and healthy leaf microbial communities by Illumina HiSeq sequencing technology. The main fungal phyla of walnut leaf-associated were Ascomycota , Basidiomycota , and Glomeromycota . Diversity indices (Shannon and Chao1 index values) of healthy leaves differed significantly in the late stages of disease onset. The results showed that the fungal species that differed considerably between the healthy and infected groups differed, and the fungal species that differed significantly between the healthy and infected groups changed with the development of the leaf disease. Critical control time points were determined by analyzing the population dynamics of pathogenic fungi. Leaf-associated microorganisms are abundant and diverse, and fungal identification and diversity studies are helpful for developing more appropriate walnut management strategies
ISSN:2544-4646
1733-1331
2544-4646
DOI:10.33073/pjm-2022-038