PacBio Full-Length Transcriptome Sequencing Reveals the Mechanism of Salt Stress Response in Sonneratia apetala

Sonneratia apetala is an essential mangrove wetland restoration tree species. Studying its molecular mechanism for salt tolerance could lay a foundation for further cultivating excellent resistant germplasm. This study used a combination of PacBio isoform sequencing (Iso-seq) and BGISEQ RNA sequenci...

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Published in:Plants (Basel) 2023-11, Vol.12 (22), p.3849
Main Authors: Chen, Beibei, Liu, Tingting, Yang, Zhuanying, Yang, Shaoxia, Chen, Jinhui
Format: Article
Language:English
Subjects:
Abiotic stress
Cell walls
Environmental restoration
Enzymatic activity
Enzyme activity
Free radicals
full-length transcriptome
Gene expression
Gene sequencing
Genes
Germplasm
Homeostasis
Kinases
Metabolism
Molecular modelling
Physiology
Plant growth
Plant species
Ribonucleic acid
RNA
Salinity tolerance
salt stress
Salt tolerance
Sequence analysis
Signal transduction
Software
Sonneratia apetala
Stress response
Transcription factors
transcriptional regulation
Transcriptomes
Yeast
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Summary:Sonneratia apetala is an essential mangrove wetland restoration tree species. Studying its molecular mechanism for salt tolerance could lay a foundation for further cultivating excellent resistant germplasm. This study used a combination of PacBio isoform sequencing (Iso-seq) and BGISEQ RNA sequencing (RNA-seq) to analyze the molecular mechanism to salt stress response of one-year-old S. apetala leaves. The growth and physiological analysis showed that physiological indexes such as growth rate, net photosynthetic rate and antioxidant enzyme activity all exhibit significant changes under salt stress. From Iso-seq, a total of 295,501 full-length transcripts, with an average length of 1418 bp, were obtained. RNA-seq produced 4712 differentially expressed genes (DEGs) as compared to a control group. Of these, 930 were identified to be co-expressed during the STEM time sequence analysis. Further, 715 and 444 co-expressed DEGs were annotated by GO and KEGG analyses, respectively. Moreover, 318 of the co-expressed DEGs were annotated as essential genes that were implicated in salt stress response of S. apetala, which were involved in transcription factors, signal transduction, hormone response, ROS homeostasis, osmotic balance, cell wall synthesis or modification. These results provide candidate targets for further characterization and offer insights into the salt-tolerant mechanism of S. apetala.
ISSN:2223-7747
2223-7747
DOI:10.3390/plants12223849