Response surface optimization of microwave-assisted extraction for HPLC-fluorescence determination of puerarin and daidzein in Radix Puerariae thomsonii

Microwave-assisted extraction was optimized with response surface methodology for HPLC-fluorescence determination of puerarin and daidzein in Radix Puerariae thomsonii.The optimized extraction procedure was achieved by soaking the sample with 70% methanol（1∶15,v/v）for 30 min,and then microwave irrad...

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Bibliographic Details
Published in:Journal of pharmaceutical analysis 2011-02, Vol.1 (1), p.13-19
Main Authors: Liu, Ying-Kun, Yan, E., Zhan, Han-Ying, Zhang, Zhi-Qi
Format: Article
Language:English
Subjects:
daidzein
HPLC-fluorescence detection
microwave-assisted extraction (MAE)
Original article
puerarin
Radix Puerariae thomsonii
response surface methodology (RSM)
响应曲面法
大豆黄酮
微波辅助提取
微波辅助萃取
荧光测定
葛根素
表面优化
高效液相色谱
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Summary:Microwave-assisted extraction was optimized with response surface methodology for HPLC-fluorescence determination of puerarin and daidzein in Radix Puerariae thomsonii.The optimized extraction procedure was achieved by soaking the sample with 70% methanol（1∶15,v/v）for 30 min,and then microwave irradiation for 11 min at a power of 600 W.Coupling the extraction process with HPLC-fluorescence presented good recovery,satisfactory precision,and good linear relation.Compared with a method from the Chinese Pharmacopoeia,the proposed method enables higher extraction efficiency and more accurate analytical results.It can be of potential value in quality assessment of Radix Puerariae thomsonii medicinal materials.
ISSN:2095-1779
2214-0883
DOI:10.1016/S2095-1779(11)70003-X